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タイトル: Exploring Cellular Targets of Vanillin Based on Morphological Changes of Saccharomyces cerevisiae
その他のタイトル: 出芽酵母の形態変化に基づくvanillinの細胞内標的探索
著者: Suga, Yohei
著者(別言語): 菅, 洋平
キーワード: Saccharomyces cerevisiae
vanillin
target-prediction
cell morphology
発行日: 2011年3月24日
抄録: Vanillin is one of the major phenolic compounds degraded from lignin. It is considered as a problematic byproduct of bioethanol production from lignocelluloses since it inhibits yeast growth and fermentation. However, detailed inhibitory mechanisms of vanillin are still unknown. In this study, I investigated intercellular targets of vanillin based on the image profiling method to infer the drug targets developed recently (Ohnuki et al., 2010). Using this method, I revealed that the morphology of wild-type cells treated with vanillin is similar with that of erg6Δ and his3Δ adh6Δ treated with vanillin. From the result of erg6Δ, the mutant of elgosterol-biosynthesis, it was suggested that vanillin does not affect its pathway. From the result of his3Δ adh6Δ, the mutant of vanillin-conversion, it was suggested that the accuracy of the image profiling was increased probably due to the inability of vanillin bio-conversion of his3Δ adh6Δ, which makes the effects of vanillin more aggravate. In common with the three strains, cell sizes were reduced in the presence of vanillin in a dose-dependent manner and significant enrichment of gene ontology term "ribosomal large subunit" was detected in the inference results. In conclusion, these results suggest that vanillin affects ribosome or related components in yeast cells and induces growth defect.
内容記述: 報告番号: ; 学位授与年月日: 2011-03-24 ; 学位の種別: 修士 ; 学位の種類: 修士(生命科学) ; 学位記番号: 修創域第3788号 ; 研究科・専攻: 新領域創成科学研究科生命科学研究系先端生命科学専攻
URI: http://hdl.handle.net/2261/48924
出現カテゴリ:025 修士論文
1222125 修士論文(生命科学研究系先端生命科学専攻)

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