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タイトル: Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice
著者: Chujo, Tetsuya
Miyamoto, Koji
Ogawa, Satoshi
Masuda, Yuka
Shimizu, Takafumi
Kishi-Kaboshi, Mitsuko
Takahashi, Akira
Nishizawa, Yoko
Minami, Eiichi
Nojiri, Hideaki
Yamane, Hisakazu
Okada, Kazunori
発行日: 2014年6月3日
出版者: PLOS
掲載誌情報: PLoS ONE 9(6): e98737.
関連URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/
抄録: WRKY transcription factors and mitogen-activated protein kinase (MAPK) cascades have been shown to play pivotal roles in the regulation of plant defense responses. We previously reported that OsWRKY53-overexpressing rice plants showed enhanced resistance to the rice blast fungus. In this study, we identified OsWRKY53 as a substrate of OsMPK3/OsMPK6, components of a fungal PAMP-responsive MAPK cascade in rice, and analyzed the effect of OsWRKY53 phosphorylation on the regulation of basal defense responses to a virulence race of rice blast fungus Magnaporthe oryzae strain Ina86-137. An in vitro phosphorylation assay revealed that the OsMPK3/OsMPK6 activated by OsMKK4 phosphorylated OsWRKY53 recombinant protein at its multiple clustered serine-proline residues (SP cluster). When OsWRKY53 was coexpressed with a constitutively active mutant of OsMKK4 in a transient reporter gene assay, the enhanced transactivation activity of OsWRKY53 was found to be dependent on phosphorylation of the SP cluster. Transgenic rice plants overexpressing a phospho-mimic mutant of OsWRKY53 (OsWRKY53SD) showed further-enhanced disease resistance to the blast fungus compared to native OsWRKY53-overexpressing rice plants, and a substantial number of defense-related genes, including pathogenesis-related protein genes, were more upregulated in the OsWRKY53SD-overexpressing plants compared to the OsWRKY53-overexpressing plants. These results strongly suggest that the OsMKK4-OsMPK3/OsMPK6 cascade regulates transactivation activity of OsWRKY53, and overexpression of the phospho-mimic mutant of OsWRKY53 results in a major change to the rice transcriptome at steady state that leads to activation of a defense response against the blast fungus in rice plants.
内容記述: UTokyo Research掲載「イネの耐病性向上に成功」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/
UTokyo Research "Enhanced disease resistance in rice plants" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/enhanced-disease-resistance-in-rice-plants/
URI: http://hdl.handle.net/2261/55990
ISSN: 1932-6203
出現カテゴリ:014 自然科学
15610 学術雑誌論文


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