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タイトル: Screening of diatoms producing domoic acid and its derivatives in the Philippines
著者: Fe Farida A. Bajarias
Kotaki, Yuichi
Juan R. Relox Jr.
Marc Lawrence J. Romero
Elsa F. Furio
Nina, Lundholm
Koike, Kazuhiko
Fukuyo, Yasuwo
Kodama, Masaaki
キーワード: domoic acid
isodomoic acid
amnesic shellfish poisoning
Pseudo-nitzschia
Nitzschia navis-varingica
diatom
Philippines
発行日: 2006年4月28日
出版者: International Coastal Research Center, Ocean Research Institute, the University of Tokyo
掲載誌情報: Coastal marine science. Vol.30No.1, 2006.4, pp. 121-129
抄録: Domoic acid is the known causative agent responsible for amnesic shellfish poisoning (ASP). Although there is only one documented ASP case in the world, there is a potential of its occurrence in Southeast Asian countries. However, limited information on domoic acid producing diatoms is available except for Nitzschia navis-varingica, which is known to produce significant levels of domoic acid. In order to obtain fundamental data on domoic acid producing diatoms, screening of Pseudo-nitzschia and Nitzschia species were primarily performed in the Philippines. Two source areas, i.e. Manila Bay and Iba estuary of Luzon Island, were selected for observation of these diatoms. Fifty eight isolates of Pseudo-nitzscia and 18 isolates of Nitzschia-like diatoms were prepared from Manila Bay and Iba estuary, respectively. These isolates were cultured and tested for the production of domoic acid and its derivatives. Pseudo-nitzscia strains did not show any signs of domoic acid production. Five out of 18 Nitzschia isolates were confirmed to produce isodomoic acids A and B. Comparison of sonication and boiling in water bath as extraction methods was investigated and results showed that both methods yielded comparable amounts of domoic acid. Stability of domoic acid extracted by boiling was also investigated and was found out to be stable at room temperature for ten days. Results implied an advantageous and convenient way of sample preparation and preservation for international transport.
URI: http://hdl.handle.net/2261/5620
ISSN: 13493000
出現カテゴリ:Coastal Marine Science
Coastal Marine Science

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