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タイトル: Flexible Sheet-Type Sensor for Noninvasive Measurement of Cellular Oxygen Metabolism on a Culture Dish
著者: Kojima, Mari
Takehara, Hiroaki
Akagi, Takanori
Shiono, Hirofumi
Ichiki, Takanori
発行日: 2015年12月1日
出版者: PLOS
掲載誌情報: Mari Kojima, Hiroaki Takehara, Takanori Akagi, Hirofumi Shiono, and Takanori Ichiki, "Flexible sheet-type sensor for noninvasive measurement of cellular oxygen metabolism on a culture dish", PLOS ONE Online Edition: 2015/12/2 (Japan time), doi:10.1371/journal.pone.0143774.
関連URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html
抄録: A novel flexible sensor was developed for the noninvasive oxygen metabolism measurement of cultivated cells and tissues. This device is composed of a transparent double-layered polymer sheet of ethylene-vinyl alcohol (EVOH) and poly(dimethylsiloxane) (PDMS) having an array of microhole structures of 90 μm diameter and 50 μm depth on its surface. All the microhole structures were equipped with a 1-μm-thick optical chemical sensing layer of platinum porphyrin-fluoropolymer on their bottom. The three-dimensional microstructures of the sensor were fabricated by a newly developed simple and low-cost production method named self-aligned hot embossing. The device was designed to be attached slightly above the cells cultivated on a dish to form a temporarily closed microspace over the target cells during measurement. Since the change in oxygen concentration is relatively fast in the microcompartmentalized culture medium, a rapid evaluation of the oxygen consumption rate is possible by measuring the phosphorescence lifetime of the platinum porphyrin-fluoropolymer. The combined use of the device and an automated optical measurement system enabled the high-throughput sensing of cellular oxygen consumption (100 points/min). We monitored the oxygen metabolism of the human breast cancer cell line MCF7 on a Petri dish and evaluated the oxygen consumption rate to be 0.72 ± 0.12 fmol/min/cell. Furthermore, to demonstrate the utility of the developed sensing system, we demonstrated the mapping of the oxygen consumption rate of rat brain slices and succeeded in visualizing a clear difference among the layer structures of the hippocampus, i.e., the cornu ammonis (CA1 and CA3) and dentate gyrus (DG).
内容記述: UTokyo Research掲載「載せるだけで細胞の酸素消費量を測定できるシート型センサ」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html
UTokyo Research "Flexible sheet-type sensor for measuring cellular oxygen metabolism" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html
URI: http://hdl.handle.net/2261/59066
ISSN: 1932-6203
出現カテゴリ:014 自然科学
1134810 学術雑誌論文


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