UTokyo Repository 東京大学
 

UTokyo Repository >
113 工学系研究科・工学部 >
48 バイオエンジニアリング専攻 >
1134810 学術雑誌論文 >

このページ(論文)をリンクする場合は次のURLを使用してください: http://hdl.handle.net/2261/59066

タイトル: Flexible Sheet-Type Sensor for Noninvasive Measurement of Cellular Oxygen Metabolism on a Culture Dish
著者: Kojima, Mari
Takehara, Hiroaki
Akagi, Takanori
Shiono, Hirofumi
Ichiki, Takanori
発行日: 2015年12月1日
出版者: PLOS
掲載誌情報: Mari Kojima, Hiroaki Takehara, Takanori Akagi, Hirofumi Shiono, and Takanori Ichiki, "Flexible sheet-type sensor for noninvasive measurement of cellular oxygen metabolism on a culture dish", PLOS ONE Online Edition: 2015/12/2 (Japan time), doi:10.1371/journal.pone.0143774.
関連URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html
http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143774
抄録: A novel flexible sensor was developed for the noninvasive oxygen metabolism measurement of cultivated cells and tissues. This device is composed of a transparent double-layered polymer sheet of ethylene-vinyl alcohol (EVOH) and poly(dimethylsiloxane) (PDMS) having an array of microhole structures of 90 μm diameter and 50 μm depth on its surface. All the microhole structures were equipped with a 1-μm-thick optical chemical sensing layer of platinum porphyrin-fluoropolymer on their bottom. The three-dimensional microstructures of the sensor were fabricated by a newly developed simple and low-cost production method named self-aligned hot embossing. The device was designed to be attached slightly above the cells cultivated on a dish to form a temporarily closed microspace over the target cells during measurement. Since the change in oxygen concentration is relatively fast in the microcompartmentalized culture medium, a rapid evaluation of the oxygen consumption rate is possible by measuring the phosphorescence lifetime of the platinum porphyrin-fluoropolymer. The combined use of the device and an automated optical measurement system enabled the high-throughput sensing of cellular oxygen consumption (100 points/min). We monitored the oxygen metabolism of the human breast cancer cell line MCF7 on a Petri dish and evaluated the oxygen consumption rate to be 0.72 ± 0.12 fmol/min/cell. Furthermore, to demonstrate the utility of the developed sensing system, we demonstrated the mapping of the oxygen consumption rate of rat brain slices and succeeded in visualizing a clear difference among the layer structures of the hippocampus, i.e., the cornu ammonis (CA1 and CA3) and dentate gyrus (DG).
内容記述: UTokyo Research掲載「載せるだけで細胞の酸素消費量を測定できるシート型センサ」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html
UTokyo Research "Flexible sheet-type sensor for measuring cellular oxygen metabolism" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/flexible-sheet-type-sensor-for-measuring-cellular-oxygen-metabolism.html
URI: http://hdl.handle.net/2261/59066
ISSN: 1932-6203
出現カテゴリ:014 自然科学
1134810 学術雑誌論文

この論文のファイル:

ファイル 記述 サイズフォーマット
journal.pone.0143774.pdf2.15 MBAdobe PDF見る/開く

本リポジトリに保管されているアイテムはすべて著作権により保護されています。

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - ご意見をお寄せください