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タイトル: Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities
著者: Ohira, Tsuyoshi
Tsutsui, Naoaki
Nagasawa, Hiromichi
Wilder, Marcy N.
著者(別言語): 長澤, 寛道
キーワード: Crustacea
eyestalk hormone
hyperglycemic activity
recombinant peptide
sinus gland
発行日: 2006年
出版者: Zoological Society of Japan
掲載誌情報: Zoological science, 23(4), 2006, pp. 383-391
抄録: Crustacean hyperglycemic hormone (CHH) is released from the X-organ/sinus gland complex located in the eyestalks, and regulates glucose levels in the hemolymph. In the giant freshwater prawn (Macrobrachium rosenbergii), two cDNAs encoding different CHH molecules were previously cloned by other workers. One of these (Mar-CHH-2) was expressed only in the eyestalks, whereas the other (Mar-CHH-L) was expressed in the heart, gills, antennal gland, and thoracic ganglion, but not in the eyestalks. However, their biological activities had not yet been characterized. Therefore, in this study, recombinant Mar-CHH-2 (rMar-CHH-2) and Mar-CHH-L (rMar-CHH-L) were produced using an E. coli expression system, by expression in bacterial cells and recovery in the insoluble fraction. Thereafter, rMar-CHH-2 and rMar-CHH-L were subjected to refolding and were subsequently purified by reversed-phase HPLC. The rMar-CHH-2 and rMar-CHH-L thus obtained exhibited the same disulfide bond arrangements as those of other CHHs reported previously, indicative of natural conformation. In in vivo bioassay, rMar-CHH-2 showed significant hyperglycemic activity, whereas rMar-CHH-L had no effect. These results indicate that Mar-CHH-L does not function as a CHH, but may have some other, unknown function.
URI: http://hdl.handle.net/2261/8078
ISSN: 02890003
出現カテゴリ:1160410 学術雑誌論文
014 自然科学


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