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タイトル: Analysis of rhizosphere bacteria of rice cultivated in Andosol lowland and upland fields using molecular biological methods
著者: Doi, Tetsuya
Hagiwara, Yusuke
Abe, Jun
Morita, Shigenori
著者(別言語): 土肥, 哲哉
阿部, 淳
森田, 茂紀
キーワード: 16S rDNA
bacteria
FISH
PCR-DGGE
rhizosphere
rice (Oryza sativa L.)
発行日: 2007年
出版者: Japanese Society for Root Research
掲載誌情報: Plant Root, vol. 1, 2007, pp. 66-74
抄録: Bacteria in the rhizosphere influence plant growth and interact with plant roots. Microscopy and culture method have been used for studies of microorganisms of the rhizosphere, but these methods are insufficient for evaluation because most rhizosphere bacteria are viable but non-culturable (VBNC). Bacteria in the rhizosphere of rice cultivated in Andosol lowland and upland fields were analyzed in this study using PCR-DGGE and FISH, in combination with modified pretreatments. Results show that the two methods with the pretreatments, more than conventional methods, provided a rapid and simple analysis of rhizosphere bacteria. The 16S rDNA band pattern of bacteria in the rhizosphere obtained using PCR-DGGE indicated different species composition of bacterial community in the two ecosystems and greater diversity of bacteria in the rhizosphere in upland field. Sequencing of major 16S rDNA bands identified Bacterium A35 and Clostridium bifermentans as dominant bacteria in the rhizosphere of rice in lowland fields and Klebsiella planticola and Bacillus fusiformis in upland fields. Furthermore, FISH observation indicated the predominance of gram-positive low GC bacteria in both rhizospheres and a higher proportion of Clostridium spp. in lowland fields, which is consistent with results of PCR-DGGE analysis. The results suggest that the bacteria in the rice rhizosphere can be changed depending on aerobic and anaerobic conditions of fields. It is expected to apply the PCR-DGGE and FISH to agricultural field experiments as reliable methods to evaluate the rhizosphere bacteria.
URI: http://hdl.handle.net/2261/8121
ISSN: 18816754
出現カテゴリ:1160210 学術雑誌論文
014 自然科学

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