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  1. 116 農学生命科学研究科・農学部
  2. 04 応用生命化学専攻
  3. 1160410 学術雑誌論文
  1. 0 資料タイプ別
  2. 10 学術雑誌論文
  3. 014 自然科学

Immunolocalization of gastrolith matrix protein (GAMP) in the gastroliths and exoskeleton of crayfish, Procambarus clarkii

http://hdl.handle.net/2261/8074
http://hdl.handle.net/2261/8074
181ad359-4fee-432c-92b9-ca48280fd20c
名前 / ファイル ライセンス アクション
ZS17_179.pdf ZS17_179.pdf (263.0 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-12-14
タイトル
タイトル Immunolocalization of gastrolith matrix protein (GAMP) in the gastroliths and exoskeleton of crayfish, Procambarus clarkii
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Takagi, Yasuaki

× Takagi, Yasuaki

WEKO 105895

Takagi, Yasuaki

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Ishii, Katsuaki

× Ishii, Katsuaki

WEKO 105896

Ishii, Katsuaki

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Ozaki, Noriaki

× Ozaki, Noriaki

WEKO 105897

Ozaki, Noriaki

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Nagasawa, Hiromichi

× Nagasawa, Hiromichi

WEKO 105898

Nagasawa, Hiromichi

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著者別名
識別子Scheme WEKO
識別子 105899
姓名 長澤, 寛道
著者所属
著者所属 Otsuchi Marine Research Center, Ocean Research Institute, The University of Tokyo
著者所属
著者所属 Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
抄録
内容記述タイプ Abstract
内容記述 Gastrolith matrix protein (GAMP) is a novel protein purified from gastroliths of the crayfish, Procambarus clarkii, and has been suggested to be associated with calcium carbonate deposition. In the present study, a specific antibody against GAMP was raised and distribution of GAMP immunoreactivity was studied in crayfish gastrolith and exoskeleton. Localization of calcium carbonate in the exoskeleton, determined by silver nitrate staining and energy dispersive X-ray microanalysis, was compared with that of GAMP immunoreactivity. Crystalline forms of calcium carbonate were also determined. SDS-PAGE and Western blotting revealed that the gastrolith extract contained a major band which was GAMP-immunopositive and showed the same mobility as that of purified GAMP. The exoskeleton extract showed smear bands, but no immunoreaction was detected. By using immunohistochemistry, the anti-GAMP antiserum reacted almost uniformly with gastrolith matrix irrespective of gastrolith size. Epithelial cells of the gastrolith disc were also immunopositive. In the exoskeleton, exocuticle was strongly GAMP-immunopositive, whereas the endocuticle and membrane layer was slightly positive. The epicuticle was immunonegative. Calcium carbonate was detected in exocuticle, endocuticle and a part of the membrane layer, but not in the epicuticle. Thus, the distribution of GAMP immunoreactivity roughly corresponded with that of calcium carbonate. X-ray diffraction study showed that calcium carbonate in the gastrolith was amorphous, whereas that in the exoskeleton consisted of calcite crystals. These data indicate that a GAMP-immunoreactive substance is commonly distributed in the mineralized tissues of the crayfish, but may exist in a chemically different form in other tissues.
書誌情報 Zoological science

巻 17, 号 2, p. 179-184, 発行日 2000
ISSN
収録物識別子タイプ ISSN
収録物識別子 02890003
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10545874
DOI
識別子タイプ DOI
関連識別子 info:doi/10.2108/zsj.17.179
フォーマット
内容記述タイプ Other
内容記述 application/pdf
日本十進分類法
主題Scheme NDC
主題 480
出版者
出版者 Zoological Society of Japan
出版者別名
日本動物学会
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