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Molecular cloning and expression analysis of a cDNAs encoding androgenic gland hormone precursors from two Porcellionidae species, Porcellio scaber and P. dilatatus
http://hdl.handle.net/2261/8075
http://hdl.handle.net/2261/8075ae0ac6d9-8648-4b29-b84f-ad04970ad55b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ZS20_75.pdf (175.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-12-14 | |||||
| タイトル | ||||||
| タイトル | Molecular cloning and expression analysis of a cDNAs encoding androgenic gland hormone precursors from two Porcellionidae species, Porcellio scaber and P. dilatatus | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | androgenic gland hormone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Crustacea | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | terrestrial isopod | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Ohira, Tsuyoshi
× Ohira, Tsuyoshi× Hasegawa, Yuriko× Okuno, Atsuro× Nagasawa, Hiromichi |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 105904 | |||||
| 姓名 | 長澤, 寛道 | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | Department of Biology, Keio University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Male sexual characteristics in Crustacea are induced by androgenic gland hormone (AGH), which is produced by the male-specific androgenic gland. Recently, AGH in the terrestrial isopod Armadillidium vulgare was characterized and its cDNA cloned, the first example in which the structure of AGH was elucidated. We report here the molecular cloning of cDNAs encoding AGH precursors from two additional terrestrial isopods, Porcellio scaber and P. dilatatus. cDNA fragments encoding Porcellio scaber AGH (Pos-AGH) and P. dilatatus AGH (Pod-AGH) were amplified by RT-PCR using degenerate oligonucleotide primers designed based on the amino acid sequence of A. vulgare AGH (Arv-AGH). Subsequently, full length cDNAs were obtained by 5'- and 3'-RACE. Both AGH precursors consisted of a signal peptide, B chain, C peptide and A chain, and exhibited the same organization as that of Arv-AGH. The amino acid sequences of the A and B chains, which comprise mature AGH peptide, were highly conserved among the three species, while that of the C peptide showed only low sequence similarity. In Northern blot analysis, each cDNA fragment used as a probe specifically hybridized with a single band (0.75 kb) in mRNA extracted from whole male reproductive organs. In analysis of the tissue-specific gene expression of these two AGHs by RT-PCR, it was revealed that both AGH transcripts were detected only in cDNA synthesized using total RNA from the testis carrying the androgenic glands, but not in that from testis only, seminal vesicle, vas deferens, or hepatopancreas. | |||||
| 書誌情報 |
Zoological science 巻 20, 号 1, p. 75-81, 発行日 2003 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 02890003 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10545874 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.2108/zsj.20.75 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 480 | |||||
| 出版者 | ||||||
| 出版者 | Zoological Society of Japan | |||||
| 出版者別名 | ||||||
| 日本動物学会 | ||||||
