Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities
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Japan International Research Center for Agricultural Sciences
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
抄録
Crustacean hyperglycemic hormone (CHH) is released from the X-organ/sinus gland complex located in the eyestalks, and regulates glucose levels in the hemolymph. In the giant freshwater prawn (Macrobrachium rosenbergii), two cDNAs encoding different CHH molecules were previously cloned by other workers. One of these (Mar-CHH-2) was expressed only in the eyestalks, whereas the other (Mar-CHH-L) was expressed in the heart, gills, antennal gland, and thoracic ganglion, but not in the eyestalks. However, their biological activities had not yet been characterized. Therefore, in this study, recombinant Mar-CHH-2 (rMar-CHH-2) and Mar-CHH-L (rMar-CHH-L) were produced using an E. coli expression system, by expression in bacterial cells and recovery in the insoluble fraction. Thereafter, rMar-CHH-2 and rMar-CHH-L were subjected to refolding and were subsequently purified by reversed-phase HPLC. The rMar-CHH-2 and rMar-CHH-L thus obtained exhibited the same disulfide bond arrangements as those of other CHHs reported previously, indicative of natural conformation. In in vivo bioassay, rMar-CHH-2 showed significant hyperglycemic activity, whereas rMar-CHH-L had no effect. These results indicate that Mar-CHH-L does not function as a CHH, but may have some other, unknown function.
雑誌名
Zoological science
巻
23
号
4
ページ
383 - 391
発行年
2006
ISSN
02890003
書誌レコードID
AA10545874
DOI
info:doi/10.2108/zsj.23.383
フォーマット
application/pdf
日本十進分類法
480
出版者
Zoological Society of Japan
出版者別名
日本動物学会
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Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities
ZS23_383.pdf
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