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Analysis of rhizosphere bacteria of rice cultivated in Andosol lowland and upland fields using molecular biological methods
http://hdl.handle.net/2261/8121
http://hdl.handle.net/2261/812185fcf315-b8b0-48d2-a5f4-ad8e09abf1bd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1_66.pdf (819.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-01-24 | |||||
| タイトル | ||||||
| タイトル | Analysis of rhizosphere bacteria of rice cultivated in Andosol lowland and upland fields using molecular biological methods | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 16S rDNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bacteria | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | FISH | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PCR-DGGE | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | rhizosphere | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | rice (Oryza sativa L.) | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Doi, Tetsuya
× Doi, Tetsuya× Hagiwara, Yusuke× Abe, Jun× Morita, Shigenori |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 858 | |||||
| 姓名 | 土肥, 哲哉 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 859 | |||||
| 姓名 | 阿部, 淳 | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 860 | |||||
| 姓名 | 森田, 茂紀 | |||||
| 著者所属 | ||||||
| 著者所属 | Field Production Science Center, Graduate School of Agriculture and Life Sciences, The University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | Faculty of Agriculture, The University of Tokyo | |||||
| 著者所属 | ||||||
| 著者所属 | AE-Bio, Graduate School of Agriculture and Life Sciences, The University of Tokyo | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Bacteria in the rhizosphere influence plant growth and interact with plant roots. Microscopy and culture method have been used for studies of microorganisms of the rhizosphere, but these methods are insufficient for evaluation because most rhizosphere bacteria are viable but non-culturable (VBNC). Bacteria in the rhizosphere of rice cultivated in Andosol lowland and upland fields were analyzed in this study using PCR-DGGE and FISH, in combination with modified pretreatments. Results show that the two methods with the pretreatments, more than conventional methods, provided a rapid and simple analysis of rhizosphere bacteria. The 16S rDNA band pattern of bacteria in the rhizosphere obtained using PCR-DGGE indicated different species composition of bacterial community in the two ecosystems and greater diversity of bacteria in the rhizosphere in upland field. Sequencing of major 16S rDNA bands identified Bacterium A35 and Clostridium bifermentans as dominant bacteria in the rhizosphere of rice in lowland fields and Klebsiella planticola and Bacillus fusiformis in upland fields. Furthermore, FISH observation indicated the predominance of gram-positive low GC bacteria in both rhizospheres and a higher proportion of Clostridium spp. in lowland fields, which is consistent with results of PCR-DGGE analysis. The results suggest that the bacteria in the rice rhizosphere can be changed depending on aerobic and anaerobic conditions of fields. It is expected to apply the PCR-DGGE and FISH to agricultural field experiments as reliable methods to evaluate the rhizosphere bacteria. | |||||
| 書誌情報 |
Plant Root 巻 1, p. 66-74, 発行日 2007 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 18816754 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.3117/plantroot.1.66 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 471.1 | |||||
| 出版者 | ||||||
| 出版者 | Japanese Society for Root Research | |||||
| 出版者別名 | ||||||
| 根研究会 | ||||||
