東京大学大学院新領域創成科学研究科環境学研究系人間環境学専攻
Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo
Abstract
Transplantation of stem cell-derived neurons into the damaged neuronal tissue is one of the promising methods for the recovery of neuronal functions. However, little is known about the signal transmission between transplanted stem cell-derived neuronal networks and the host brain tissue during recovery. In this study, we developed microelectrode-array (MEA) substrates with multiple micro-chambers for cell cultures. Each micro-chamber consisted of 4 cell-plating spots with interconnecting conduits fabricated by polydimethylsiloxane (PDMS) lithography. The conduits had the tunneling structures, the height of which was approximately 6 μm, through which only neurites could grow into adjacent cell-plating spots. We cultured mouse cortical and stem cell-derived neurons in the subset of cell-plating spots. Here, we discussed this co-culture device utility for monitoring of transplantation processes in vitro.
発行年
2011-03-24
日本十進分類法
491
学位名
修士(科学)
学位
master
研究科・専攻
新領域創成科学研究科環境学研究系人間環境学専攻
学位授与年月日
2011-03-24
学位記番号
修創域第3959号
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分化誘導細胞 : 生体由来細胞系共培養デバイスの開発(要旨)
要旨(summary)
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