Research Center for Oceanography-LIPI
School of Marine Biosciences Kitasato University
Natural Museum of Denmark, University of Copenhagen
University Malaysia Sarawak
The University of Tokyo
抄録
In order to explore the detailed distribution of N. navis-varingica that produces ASP toxins in Asian waters, a screening was made in four areas of Indonesia and a part of Malaysia. Diatom samples were collected by a plankton net. Nitzschia-like diatoms were isolated from crude pre-cultures and cultured in f/2 medium followed by HPLC-fluorescence analysis. Unlike other Asian countries, Nitzschia navis-varingica-like diatoms were rare in Indonesian and Malaysian samples, which is partly attributable to the extreme pH and salinity at some of the localities. Fifteen, six and eleven isolates were obtained from South Sulawesi, Lampung and Sangihe Island in Indonesia, respectively, none from Jakarta Bay, Indonesia and eight strains from Kota Kinabalu, Malaysia. All of the fifteen strains from South Sulawesi (Panyula) produced toxins. They produced on average 2.7 pg toxin cell(-1) with the toxin profile of DA : IB (98 : 2) similar to results on strains from Thailand and northern Japan. This toxin production was confirmed by LC-MS/MS analysis. These toxin-producing strains were identified as N. navis-varingica. Eleven sub-strains of a single parental strain showed the same toxin profile (on average 1.1 pg toxin cell(-1), DA: IB=94 : 6). The remaining Nitzschia strains did not produce ASP toxins. Twenty isolates of Pseudo-nitzschia from Lampung Bay were not found to produce ASP toxins.
内容記述
Proceedings of the Horiba International Conference "New Direction of Ocean Research in the Western Pacific" : Past, Present and Future of UNESCO/IOC/WESTPAC Activity for 50 years and the JSPS Project "Coastal Marine Science"
Section I: Research Articles/Harmful microalgae
雑誌名
Coastal marine science
巻
35
号
1
ページ
34 - 38
発行年
2012-03-20
ISSN
13493000
書誌レコードID
AA11957899
日本十進分類法
452
出版者
International Coastal Research Center, Atmosphere and Ocean Research Institute, the University of Tokyo
Screening of diatoms that produce ASP toxins in Southernmost Asian waters
CMS350105.pdf
(663.38KB)
