東京大学大学院農学生命科学研究科生物材料科学専攻
Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
東京大学大学院農学生命科学研究科附属演習林樹芸研究所
Arboricultural Research Institute, The University of Tokyo Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo
抄録
We investigated 80% methanol-soluble leaf extracts (predominantly phenolics) from 2-3 year old Eucalyptus saligna (Es) leaves by colorimetric assay and HPLC. In the species E. saligna, a different line number was given depending on the production area. The flavanol content in Es 20483 leaf was clearly correlated to the leaf height from the ground, although other Es lines were not. Furthermore, PAL-like gene sequence was analyzed based on genomic DNA and mRNA extracted from leaves of three lines of Es. A total of 32 PAL-like gene sequences could be cloned from 3 Es lines, and among them, activity at the crude enzyme solution level was confirmed. Phylogenetic analysis showed that one clade was formed with 20483 PALs and 20483 strains had different evolutionary features from the other 2 lines. Within the Es species, there were differences in the mechanism of production and accumulation of phenolic components, and gene sequence depending on the lineage.
雑誌名
東京大学農学部演習林報告 = Bulletin of The University of Tokyo Forests
Eucalyptus saligna の葉に含まれるフェノール性成分とその生合成に関わるPAL酵素の解析
esrh138001.pdf
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