WEKO3
アイテム
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Establishment of a Tissue Culture System of Populus euphratica Oliv
http://hdl.handle.net/2261/22494
http://hdl.handle.net/2261/22494297e692d-b285-41f4-b763-e1294b9dd6d4
名前 / ファイル | ライセンス | アクション |
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esrh102003.pdf (1.0 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2014-09-03 | |||||
タイトル | ||||||
タイトル | Establishment of a Tissue Culture System of Populus euphratica Oliv | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Populus euphratica | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | 組織培養 | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | 植物体再生 | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | えき芽 | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | 継代培養 | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Populus euphratica | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | tissue culture | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | plant regeneration | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | axillary buds | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | subculture | |||||
主題Scheme | Other | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Popu Iuseuphratica Oiiv. の組織培養系の確立 | |||||
著者 |
WATANABE, Shin
× WATANABE, Shin× KOJIMA, Katsumi× IDE, Yuji× SASAKI, Satohiko |
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著者別名 | ||||||
識別子 | 54345 | |||||
識別子Scheme | WEKO | |||||
姓名 | 渡辺, 信 | |||||
著者別名 | ||||||
識別子 | 54346 | |||||
識別子Scheme | WEKO | |||||
姓名 | 小島, 克己 | |||||
著者別名 | ||||||
識別子 | 54347 | |||||
識別子Scheme | WEKO | |||||
姓名 | 井出, 雄二 | |||||
著者別名 | ||||||
識別子 | 54348 | |||||
識別子Scheme | WEKO | |||||
姓名 | 佐々木, 恵彦 | |||||
著者所属 | ||||||
著者所属 | Department of Forest Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo | |||||
著者所属 | ||||||
著者所属 | Asian Natural Environmental Science Center, The University of Tokyo | |||||
著者所属 | ||||||
著者所属 | University Forests in Chiba, Faculty of Agriculture, The University of Tokyo | |||||
著者所属 | ||||||
著者所属 | College of Bioresource Sciences, Nihon University | |||||
著者所属 | ||||||
著者所属 | 東京大学大学院農学生命科学研究科 | |||||
著者所属 | ||||||
著者所属 | 東京大学アジア生物資源環境研究センター | |||||
著者所属 | ||||||
著者所属 | 東京大学農学部付属千葉演習林 | |||||
著者所属 | ||||||
著者所属 | 日本大学生物資源科学部 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Populus euphraticaの標準的な培養系を確立した。温室内における水耕栽培によって育てたP. euphraticaの苗からえき芽のついた茎軸の小片を切り分け,表面殺菌後BAPを添加したMSあるいは1/2MS培地に植え付けた。その結果,1/2MSにBAPを0.25mg/l単独で,あるいは0.05mg/lのNAAとともに添加した培地が,シュートの伸長とマルチプルシュートの形成に最も適していた。0.8mg/lのBAPを添加した1/2MS培地で一旦継代培養した後,継代培養に適した培地条件を検討した。その結果,0.2mg/lのBAPを添加した培地でもっともガラス化するシュートの割合が少なく健全なシュートが得られた。BAPを含まない培地ではNAAの添加の有無に関わらずシュートからの発根が認められた。NAAを0.01mg/l添加した場合には発根率がもっとも高く90%に達した。得られた植物体は,ほぼ100%順化が可能であり,温室内に置いて3ヶ月後には50~80cmの苗木に育った。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A standard tissue culture system of Populus euphratica was established. Stem segments with axillary buds were excised from seedlings grown hydroponically in a greenhouse. They were surface-sterilized and cultured on MS or 1/2MS medium supplemented with various concentrations of BAP and NAA. Among the tested media, 1/2MS with 0.25mg/l of BAP alone or in combination with 0.05mg/l of NAA were optimal for shoot elongation and multiple shoot formation. After subculture on 1/2MS with 0.8mg/l of BAP, the optimal BAP and NAA concentration was surveyed for rapid clonal propagation. Shoots grew most healthily and the rate of vitrification was the lowest on the 1/2MS medium supplemented with 0.2mg/l of BAP. Rooting occurred on media without BAP irrespective of NAA concentration. The ratio of rooted shoots increased up to the highest of 90% at the concentration of 0.01mg/l NAA after three weeks of culture. Almost all plantlets were successfully acclimatized and grew into 50-80cm tall saplings in a greenhouse three months after transplanting to the potting soil. | |||||
書誌情報 |
東京大学農学部演習林報告 巻 102, p. 87-92, 発行日 1999 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03716007 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00162451 | |||||
日本十進分類法 | ||||||
主題 | 610 | |||||
主題Scheme | NDC | |||||
出版者 | ||||||
出版者 | 東京大学大学院農学生命科学研究科附属演習林 | |||||
出版者別名 | ||||||
The Tokyo University Forests |