WEKO3
アイテム
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Growth of Suspension Cultured Cell of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii×Populus plantierensis in NaCl Containing Medium
http://hdl.handle.net/2261/22499
http://hdl.handle.net/2261/22499b7061dfa-9636-4bbc-87dd-25e23460770d
名前 / ファイル | ライセンス | アクション |
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esrh102008.pdf (563.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2014-09-03 | |||||
タイトル | ||||||
タイトル | Growth of Suspension Cultured Cell of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii×Populus plantierensis in NaCl Containing Medium | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | NaClストレス | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | 培養細胞 | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | カルス再生 | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Populus euphratica | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Populus alba cv. Pyramidalis | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Populus maximowiczii×Populus plantierensis | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | NaCl stress | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Cell suspension culture | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Callus regeneration | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Populus euphratica | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Populus alba cv. Pyramidalis | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | Populus maximowiczii×Populus plantierensis | |||||
主題Scheme | Other | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | NaCI を含む培地における Populuse uphratica,Populus albacv.Pyramidalis, Populus maximowiczii× Populus plantierensis の培養細胞の成長 | |||||
著者 |
SHEN, Hailong
× SHEN, Hailong× WATANABE, Shin× IDE, Yuji |
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著者別名 | ||||||
識別子 | 54378 | |||||
識別子Scheme | WEKO | |||||
姓名 | 沈, 海龍 | |||||
著者別名 | ||||||
識別子 | 54379 | |||||
識別子Scheme | WEKO | |||||
姓名 | 渡辺, 信 | |||||
著者別名 | ||||||
識別子 | 54380 | |||||
識別子Scheme | WEKO | |||||
姓名 | 井出, 雄二 | |||||
著者所属 | ||||||
著者所属 | Research Division of the University Forests, Faculty of Agriculture, The University of Tokyo | |||||
著者所属 | ||||||
著者所属 | Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo | |||||
著者所属 | ||||||
著者所属 | Faculty of Forest Resources and Environment, Northeast Forestry University | |||||
著者所属 | ||||||
著者所属 | 東京大学農学部附属演習林研究部 | |||||
著者所属 | ||||||
著者所属 | 東京大学大学院農学生命科学研究科森林科学専攻 | |||||
著者所属 | ||||||
著者所属 | 中国東北林業大学森林資源与環境学院 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Populus euphratica, Populus alba cv. Pyramidalis, Populus maximowiczii×Populus plantierensisの3種のポプラの培養細胞について,培地へのNaClの添加量を徐々に増加させた場合の耐性を評価した。NaClを添加した最初の培養では,P. euphratica, P. maximowiczii×P. plantierensisの細胞は,200mMのNaCl添加では成長が著しく抑制された。また,P. alba cv. Pyramidalisの細胞では150mMのNaCl添加で成長が抑制された。しかし,培養を繰り返すうちに細胞はNaClに対する適応性を発達させ,6回目の継代培養終了時には,P. euphraticaの細胞は200mM, P. alba cv. Pyramidalisの細胞は150mMのNaCl添加培地でも良好な成長が可能になった。しかし,P. maximowiczii×P. plantierensisの細胞は,100mMまででしか良好な成長を示さなくなった。高NaCl条件下において種ごとの細胞成長に違いが見られたが,すべての種において9回目の継代培養において200mMのNaCl条件下での細胞の生存が確認された。また,NaCl200mMで培養された細胞は,5回から6回目の継代培養後でもカルスの再生能力を有していた。特に,P. euphraticaではNaCl250mMの条件で培養した細胞からもカルスが再生した。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Tolerance for gradually increasing NaCl stress was evaluated for the suspension cultured cells of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii×Populus plantierensis. In the first culture in a medium containing more than 200 mM NaCl, growth of P. euphratica and P. maximowiczii×P. plantierensis cells was remarkably suppressed. Growth of P. alba cv. Pyramidalis cells was suppressed in a medium containing more than 150mM NaCl. During the subculture, P. euphratica and in P. alba cv. Pyramidalis cells developed their adaptability for NaCl stress. At the end of the sixth subculture, P. euphratica could grow well in a medium containing 200mM NaCl and P. alba cv. Pyramidalis could grow well in a medium containing 150mM NaCl. On the other hand, P. maximowiczii×P. plantierensis reduced its adaptability for NaCl concentration to 100mM. Although there were differences between species in their ability of cell growth under high NaCl concentration, cells could survive in 200mM NaCl after the ninth subculture in every species. Particularly P. euphratica could divide even in a medium containing 250mM NaCl. Such surviving cells still have the ability for callus regeneration after the fifth or sixth subculture. | |||||
書誌情報 |
東京大学農学部演習林報告 巻 102, p. 143-150, 発行日 1999 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03716007 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00162451 | |||||
日本十進分類法 | ||||||
主題 | 610 | |||||
主題Scheme | NDC | |||||
出版者 | ||||||
出版者 | 東京大学大学院農学生命科学研究科附属演習林 | |||||
出版者別名 | ||||||
The Tokyo University Forests |