2024-03-29T09:51:59Z
https://repository.dl.itc.u-tokyo.ac.jp/oai
oai:repository.dl.itc.u-tokyo.ac.jp:00000169
2022-12-19T03:41:10Z
27:38:39
9:10:11
Immunohistochemical identification of hyperglycemic hormone- and molt-inhibiting hormone-producing cells in the eyestalk of the kuruma prawn, Penaeus japonicus
Shih, Tung-Wei
105878
Suzuki, Yuzuru
105879
Nagasawa, Hiromichi
105880
Aida, Katsumi
105881
480
application/pdf
This study deals with the localization of crustacean hyperglycemic hormone (CHH, Pej-SGP-III) and molt-inhibiting hormone (MIH, Pej-SGP-IV) in the eyestalk of the kuruma prawn Penaeus japonicus using immunohistochemistry. High-titer and highly specific antisera were raised in rabbits against synthetic Pej-SGP-III C-terminal peptide (Glu-Glu-His-Met-Ala-Ala-Met-Gln-Thr-Val-NH2) and Pej-SGP-IV C-terminal peptide (Val-Trp-Ile-Ser-Ile-Leu-Asn-Ala-Gly-Gln-OH), both of which were conjugated with bovine serum albumin by a cross linker. Eyestalks were removed from mature male prawns at the intermolt stage of the molting cycle and fixed in Bouin's solution. Serial sections stained immunohistochemically showed that neurosecretory cells of Pej-SGP-III and Pej-SGP-IV were located in the same cluster of the medulla terminalis ganglionic X-organ (MTGX), and that three kinds of neurosecretory cells, which were stained with anti-Pej-SGP-III antiserum and/or anti-Pej-SGP-IV antiserum were present. The number of neurosecretory cells which stained with both antisera was much fewer than that of neurosecretory cells which stained with one of the antisera only. The axon and axon terminals in the sinus gland were also stained and the staining density of the sinus gland was always deeper than that of the neurosecretory cells.
journal article
Zoological Society of Japan
1998
application/pdf
Zoological science
3
15
389
397
AA10545874
02890003
https://repository.dl.itc.u-tokyo.ac.jp/record/169/files/ZS15_389.pdf
eng
info:doi/10.2108/zsj.15.389