2024-03-28T17:50:07Z
https://repository.dl.itc.u-tokyo.ac.jp/oai
oai:repository.dl.itc.u-tokyo.ac.jp:00000665
2022-12-19T03:41:41Z
75:151:152
9:10:11
Reconstruction of Insulin Signal Flow from Phosphoproteome and Metabolome Data
Yugi, Katsuyuki
2340
Kubota, Hiroyuki
2341
Toyoshima, Yu
2342
Noguchi, Rei
2343
Kawata, Kentaro
2344
Komori, Yasunori
2345
Uda, Shinsuke
2346
Kunida, Katsuyuki
2347
Tomizawa, Yoko
2348
Funato, Yosuke
2349
Miki, Hiroaki
2350
Matsumoto, Masaki
2351
Nakayama, Keiichi I.
2352
Kashikura, Kasumi
2353
Endo, Keiko
2354
Ikeda, Kazutaka
2355
Soga, Tomoyoshi
2356
Kuroda, Shinya
2357
Cellular homeostasis is regulated by signals through multiple molecular networks that include protein phosphorylation and metabolites. However, where and when the signal flows through a network and regulates homeostasis has not been explored. We have developed a reconstruction method for the signal flow based on time-course phosphoproteome and metabolome data, using multiple databases, and have applied it to acute action of insulin, an important hormone for metabolic homeostasis. An insulin signal flows through a network, through signaling pathways that involve 13 protein kinases, 26 phosphorylated metabolic enzymes, and 35 allosteric effectors, resulting in quantitative changes in 44 metabolites. Analysis of the network reveals that insulin induces phosphorylation and activation of liver-type phosphofructokinase 1, thereby controlling a key reaction in glycolysis. We thus provide a versatile method of reconstruction of signal flow through the network using phosphoproteome and metabolome data.
UTokyo Research掲載「細胞内のビッグデータから大規模ネットワークの再構築に成功」URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/reconstruction-of-molecular-network-from-cellular-big-data/
UTokyo Research "Reconstruction of molecular network from cellular big data" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/reconstruction-of-molecular-network-from-cellular-big-data/
journal article
Elsevier
2014-08-21
application/pdf
Cell Reports
4
8
1171
1183
2211-1247
https://repository.dl.itc.u-tokyo.ac.jp/record/665/files/1-s2.0-S2211124714005907-main.pdf
eng
info:doi/10.1016/j.celrep.2014.07.021
http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/reconstruction-of-molecular-network-from-cellular-big-data/
http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/reconstruction-of-molecular-network-from-cellular-big-data/
http://www.sciencedirect.com/science/article/pii/S2211124714005907
© 2014 The Authors; This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).