2024-03-28T13:38:06Z
https://repository.dl.itc.u-tokyo.ac.jp/oai
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2022-12-19T03:41:39Z
75:159:160
9:10:11
An orthogonal ribosome-tRNAs pair via engineering of the peptidyl transferase center
Terasaka, Naohiro
2397
Hayashi, Gosuke
2398
Katoh, Takayuki
2399
Suga, Hiroaki
2400
The Watson-Crick base pairs between the 3'-terminal end of tRNAs and ribosomal RNA in the peptidyl transferase center are universally conserved. Here, we report that the introduction of compensatory mutations to Escherichia coli RNAs in this site leads to an orthogonal system independent of the wild-type counterpart, as demonstrated via the production of two peptide sequences from a single mRNA. This work thus identifies a new way to reprogram the genetic code.
UTokyo Research掲載「1つの遺伝子から2種類のペプチドが同時に翻訳合成できる?!」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/simultaneous-translation-of-two-distinct-peptides-from-single-mrna-template/
UTokyo Research "Simultaneous translation of two distinct peptides from single mRNA template" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/simultaneous-translation-of-two-distinct-peptides-from-single-mrna-template/
journal article
Nature Publishing Group
2014-06-09
application/pdf
Nature chemical biology
10
555
557
AA11994319
1552-4450
1552-4469
https://repository.dl.itc.u-tokyo.ac.jp/record/673/files/nchembio.1549_author_version.pdf
eng
info:doi/10.1038/nchembio.1549
http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/simultaneous-translation-of-two-distinct-peptides-from-single-mrna-template/
http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/simultaneous-translation-of-two-distinct-peptides-from-single-mrna-template/
http://www.nature.com/nchembio/journal/v10/n7/abs/nchembio.1549.html
Copyright (C) 2014, Rights Managed by Nature Publishing Group