2024-03-28T09:43:09Z
https://repository.dl.itc.u-tokyo.ac.jp/oai
oai:repository.dl.itc.u-tokyo.ac.jp:00000682
2022-12-19T03:41:40Z
6:167:168
9:10:11
High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition
Watanabe, Rikiya
2459
Soga, Naoki
2460
Yamanaka, Tomoko
2461
Noji, Hiroyuki
2462
We present a micro-device in which more than 10,000 asymmetric lipid bilayer membranes are formed at a time on micro-chamber arrays. The arrayed asymmetric lipid bilayers, where lipid compositions are different between the inner and outer leaflets, are formed with high efficiency of over 97% by injecting several types of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces. The lipid compositional asymmetry is an intrinsic property of bio-membranes, and therefore, this micro-device extends the versatility of artificial lipid-bilayer systems, which were previously limited to symmetric bilayer formation, and could contribute to the understanding of the role of lipid compositional asymmetry in cell physiology and also to further analytical and pharmacological applications.
UTokyo Research掲載「脂質組成の非対称性をもつ人工生体膜の新しい量産技術」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/novel-high-throughput-micro-device-to-form-asymmetric-bio-membranes/
UTokyo Research "Novel high-throughput micro-device to form asymmetric bio-membranes" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/novel-high-throughput-micro-device-to-form-asymmetric-bio-membranes/
journal article
Nature Publishing Group
2014-11-17
application/pdf
Scientific Repoorts
4
7076
2045-2322
https://repository.dl.itc.u-tokyo.ac.jp/record/682/files/srep07076.pdf
eng
info:doi/10.1038/srep07076
http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/novel-high-throughput-micro-device-to-form-asymmetric-bio-membranes/
http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/novel-high-throughput-micro-device-to-form-asymmetric-bio-membranes/
http://www.nature.com/srep/2014/141117/srep07076/full/srep07076.html
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/