2024-03-29T08:20:26Z
https://repository.dl.itc.u-tokyo.ac.jp/oai
oai:repository.dl.itc.u-tokyo.ac.jp:00040771
2022-12-19T04:16:23Z
40:6494:7162:7200:7204
9:504:7165:7202:7205
Screening of diatoms producing domoic acid and its derivatives in the Philippines
Fe, Farida A. Bajarias
139519
Kotaki, Yuichi
139520
Juan, R. Relox Jr.
139521
Marc, Lawrence J. Romero
139522
Elsa, F. Furio
139523
Nina, Lundholm
139524
Koike, Kazuhiko
139525
Fukuyo, Yasuwo
139526
Kodama, Masaaki
139527
452
domoic acid
isodomoic acid
amnesic shellfish poisoning
Pseudo-nitzschia
Nitzschia navis-varingica
diatom
Philippines
application/pdf
Domoic acid is the known causative agent responsible for amnesic shellfish poisoning (ASP). Although there is only one documented ASP case in the world, there is a potential of its occurrence in Southeast Asian countries. However, limited information on domoic acid producing diatoms is available except for Nitzschia navis-varingica, which is known to produce significant levels of domoic acid. In order to obtain fundamental data on domoic acid producing diatoms, screening of Pseudo-nitzschia and Nitzschia species were primarily performed in the Philippines. Two source areas, i.e. Manila Bay and Iba estuary of Luzon Island, were selected for observation of these diatoms. Fifty eight isolates of Pseudo-nitzscia and 18 isolates of Nitzschia-like diatoms were prepared from Manila Bay and Iba estuary, respectively. These isolates were cultured and tested for the production of domoic acid and its derivatives. Pseudo-nitzscia strains did not show any signs of domoic acid production. Five out of 18 Nitzschia isolates were confirmed to produce isodomoic acids A and B. Comparison of sonication and boiling in water bath as extraction methods was investigated and results showed that both methods yielded comparable amounts of domoic acid. Stability of domoic acid extracted by boiling was also investigated and was found out to be stable at room temperature for ten days. Results implied an advantageous and convenient way of sample preparation and preservation for international transport.
departmental bulletin paper
International Coastal Research Center, Ocean Research Institute, the University of Tokyo
2006-04-28
application/pdf
Coastal marine science
1
30
121
129
AA11957899
13493000
https://repository.dl.itc.u-tokyo.ac.jp/record/40771/files/KJ00004354593.pdf
eng