{"created":"2021-03-01T06:16:42.284761+00:00","id":170,"links":{},"metadata":{"_buckets":{"deposit":"643e9f4a-7471-45ca-8c8b-363d7f841ef8"},"_deposit":{"id":"170","owners":[],"pid":{"revision_id":0,"type":"depid","value":"170"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00000170"},"item_2_biblio_info_7":{"attribute_name":"\u66f8\u8a8c\u60c5\u5831","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"937","bibliographicPageStart":"931","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"Zoological science"}]}]},"item_2_description_13":{"attribute_name":"\u30d5\u30a9\u30fc\u30de\u30c3\u30c8","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"\u6284\u9332","attribute_value_mlt":[{"subitem_description":"We have purified and characterized two molecular forms of insulin from the Brockmann bodies of barfin flounder, Verasper moseri: a normal type of insulin (insulin-I), which consisted of 21 amino acid residues for the A-chain and 30 residues for the B-chain, and a novel type of insulin (insulin-II), which had an extension of two amino acid residues at the N-terminus of the B-chain. The additional two residues at the N-terminus of B-chain of insulin-II were Pyr-Ala which had not yet been reported in vertebrate insulins. Except for these two residues, the amino acid sequence of insulin-II was completely identical with that of insulin-I. Each Brockmann body extract from five individuals contained both insulins, indicating that insulin-I and -II were the products of non-allelic expression. By polymerase chain reaction, only one nucleotide sequence of preproinsulin gene encoding insulin-I and -II was obtained, and the amino acid sequence of A- and B-chains deduced from the nucleotide sequence was identical with that of insulin-I and also insulin-II established by Edman degradation. Furthermore, genomic Southern blot analysis using a part of nucleotide sequence of the preproinsulin gene as a probe showed a single positive band in all cases of genomic DNA digested with each of three restriction endonucleases. These results indicate that insulin-I and -II of barfin flounder arise from a single preproinsulin by proteolytic cleavage at different sites of the signal peptide region.","subitem_description_type":"Abstract"}]},"item_2_full_name_3":{"attribute_name":"\u8457\u8005\u5225\u540d","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"105885","nameIdentifierScheme":"WEKO"}],"names":[{"name":"\u9577\u6fa4, \u5bdb\u9053"}]}]},"item_2_publisher_20":{"attribute_name":"\u51fa\u7248\u8005","attribute_value_mlt":[{"subitem_publisher":"Zoological Society of Japan"}]},"item_2_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.2108/zsj.15.931","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_10":{"attribute_name":"\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID","attribute_value_mlt":[{"subitem_source_identifier":"AA10545874","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02890003","subitem_source_identifier_type":"ISSN"}]},"item_2_subject_15":{"attribute_name":"\u65e5\u672c\u5341\u9032\u5206\u985e\u6cd5","attribute_value_mlt":[{"subitem_subject":"480","subitem_subject_scheme":"NDC"}]},"item_2_text_21":{"attribute_name":"\u51fa\u7248\u8005\u5225\u540d","attribute_value_mlt":[{"subitem_text_value":"\u65e5\u672c\u52d5\u7269\u5b66\u4f1a"}]},"item_2_text_4":{"attribute_name":"\u8457\u8005\u6240\u5c5e","attribute_value_mlt":[{"subitem_text_value":"Hokkaido National Fisheries Research Institute"},{"subitem_text_value":"Department of Applied Biochemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"\u8457\u8005","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Andoh, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"105883","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagasawa, Hiromichi"}],"nameIdentifiers":[{"nameIdentifier":"105884","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"\u30d5\u30a1\u30a4\u30eb\u60c5\u5831","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-26"}],"displaytype":"detail","filename":"ZS15_931.pdf","filesize":[{"value":"867.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ZS15_931.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/170/files/ZS15_931.pdf"},"version_id":"fbed1b7d-571b-4918-adb0-2ed61c946609"}]},"item_language":{"attribute_name":"\u8a00\u8a9e","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"\u8cc7\u6e90\u30bf\u30a4\u30d7","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene","item_titles":{"attribute_name":"\u30bf\u30a4\u30c8\u30eb","attribute_value_mlt":[{"subitem_title":"Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene"}]},"item_type_id":"2","owner":"1","path":["9/10/11","27/38/39"],"pubdate":{"attribute_name":"\u516c\u958b\u65e5","attribute_value":"2007-12-14"},"publish_date":"2007-12-14","publish_status":"0","recid":"170","relation_version_is_last":true,"title":["Two molecular forms of insulin from barfin flounder, Verasper moseri, are derived from a single gene"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2021-03-02T00:45:15.920551+00:00"}