{"created":"2021-03-01T06:16:42.704871+00:00","id":177,"links":{},"metadata":{"_buckets":{"deposit":"966dcd96-9f98-4822-a00e-1dfd951631ad"},"_deposit":{"id":"177","owners":[],"pid":{"revision_id":0,"type":"depid","value":"177"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00000177","sets":["27:38:39","9:10:11"]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"391","bibliographicPageStart":"383","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"Zoological science"}]}]},"item_2_description_13":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Crustacean hyperglycemic hormone (CHH) is released from the X-organ/sinus gland complex located in the eyestalks, and regulates glucose levels in the hemolymph. In the giant freshwater prawn (Macrobrachium rosenbergii), two cDNAs encoding different CHH molecules were previously cloned by other workers. One of these (Mar-CHH-2) was expressed only in the eyestalks, whereas the other (Mar-CHH-L) was expressed in the heart, gills, antennal gland, and thoracic ganglion, but not in the eyestalks. However, their biological activities had not yet been characterized. Therefore, in this study, recombinant Mar-CHH-2 (rMar-CHH-2) and Mar-CHH-L (rMar-CHH-L) were produced using an E. coli expression system, by expression in bacterial cells and recovery in the insoluble fraction. Thereafter, rMar-CHH-2 and rMar-CHH-L were subjected to refolding and were subsequently purified by reversed-phase HPLC. The rMar-CHH-2 and rMar-CHH-L thus obtained exhibited the same disulfide bond arrangements as those of other CHHs reported previously, indicative of natural conformation. In in vivo bioassay, rMar-CHH-2 showed significant hyperglycemic activity, whereas rMar-CHH-L had no effect. These results indicate that Mar-CHH-L does not function as a CHH, but may have some other, unknown function.","subitem_description_type":"Abstract"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"105917","nameIdentifierScheme":"WEKO"}],"names":[{"name":"長澤, 寛道"}]}]},"item_2_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Zoological Society of Japan"}]},"item_2_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.2108/zsj.23.383","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10545874","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02890003","subitem_source_identifier_type":"ISSN"}]},"item_2_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"480","subitem_subject_scheme":"NDC"}]},"item_2_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"日本動物学会"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Japan International Research Center for Agricultural Sciences"},{"subitem_text_value":"Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ohira, Tsuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"105913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsutsui, Naoaki"}],"nameIdentifiers":[{"nameIdentifier":"105914","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagasawa, Hiromichi"}],"nameIdentifiers":[{"nameIdentifier":"105915","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wilder, Marcy N."}],"nameIdentifiers":[{"nameIdentifier":"105916","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-26"}],"displaytype":"detail","filename":"ZS23_383.pdf","filesize":[{"value":"479.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ZS23_383.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/177/files/ZS23_383.pdf"},"version_id":"7d985e81-7fe3-4c15-8437-8d82de61ce04"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Crustacea","subitem_subject_scheme":"Other"},{"subitem_subject":"eyestalk hormone","subitem_subject_scheme":"Other"},{"subitem_subject":"hyperglycemic activity","subitem_subject_scheme":"Other"},{"subitem_subject":"recombinant peptide","subitem_subject_scheme":"Other"},{"subitem_subject":"sinus gland","subitem_subject_scheme":"Other"},{"subitem_subject":"X-organ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities"}]},"item_type_id":"2","owner":"1","path":["11","39"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-12-14"},"publish_date":"2007-12-14","publish_status":"0","recid":"177","relation_version_is_last":true,"title":["Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:41:08.877807+00:00"}