{"created":"2021-03-01T06:43:10.485893+00:00","id":25232,"links":{},"metadata":{"_buckets":{"deposit":"983389c9-e0a3-4124-8172-9b3a52693742"},"_deposit":{"id":"25232","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25232"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025232","sets":["27:2466:2467:2483","9:504:2469:2484"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"裸子植物におけるMADS-box遺伝子群の系統解析"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-07-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"24","bibliographicPageStart":"15","bibliographicVolumeNumber":"129","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"被子植物においては花器官形成に関わるMADS-box遺伝子群が知られている。本研究は裸子植物における雌雄の器官形成の進化を知ることを目的とし，MADS-box遺伝子の部分配列を決定し，系統解析を行った。対象植物は，裸子植物であるイチョウ，グネツム，マオウ，ソテツ，エゾマツ，ドイツトウヒ，イチイ，スギ，メタセコイア，およびシダ植物であるヒメワラビの計10種であった。実験手法は，裸子植物（イチョウ，グネツム，トウヒ，スギ）で既に知られているMADS-box遺伝子の保存領域からデジェネレートプライマーを設計し，10種の葉から抽出したDNAをテンプレートとして計91組のプライマー対でPCRを行った。増幅されたDNA断片はクローニング後選抜し，塩基配列を決定した。その結果，イチョウ，ソテツ，エゾマツの3種において，MADS-box遺伝子の部分配列86～87塩基長がそれぞれ2，3，4種類得られた。このうちイチョウから得た2種類の配列は，既に報告されている配列（GBM21，GBM30）と一致したが，それぞれ新たに延長する配列を決定した。またソテツ，エゾマツからそれぞれ得た2種類のMADS-box配列は，機能の知られる既存のMADS-box遺伝子のホモログであることが推測された。特に今回新たに決定したソテツのMADS-box配列は，花器官の進化を探る上で今後重要な情報になると考えられる。","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"53414","nameIdentifierScheme":"WEKO"}],"names":[{"name":"会沢, 栄志"}]},{"nameIdentifiers":[{"nameIdentifier":"53415","nameIdentifierScheme":"WEKO"}],"names":[{"name":"兼行, 民治郎"}]},{"nameIdentifiers":[{"nameIdentifier":"53416","nameIdentifierScheme":"WEKO"}],"names":[{"name":"寺田, 珠実"}]},{"nameIdentifiers":[{"nameIdentifier":"53417","nameIdentifierScheme":"WEKO"}],"names":[{"name":"鮫島, 正浩"}]},{"nameIdentifiers":[{"nameIdentifier":"53418","nameIdentifierScheme":"WEKO"}],"names":[{"name":"鴨田, 重裕"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The University of Tokyo Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Japan International Cooperation Agency"},{"subitem_text_value":"Nippon Paper Industries Co., Ltd."},{"subitem_text_value":"Arboricultural Research Institute, The University of Tokyo Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"独立行政法人国際協力機構"},{"subitem_text_value":"日本製紙株式会社"},{"subitem_text_value":"東京大学大学院農学生命科学研究科附属演習林樹芸研究所"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Aizawa, Eishi"}],"nameIdentifiers":[{"nameIdentifier":"53409","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kaneyuki, Tamijiro"}],"nameIdentifiers":[{"nameIdentifier":"53410","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Terada, Tamami"}],"nameIdentifiers":[{"nameIdentifier":"53411","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Samejima, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"53412","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamoda, Shigehiro"}],"nameIdentifiers":[{"nameIdentifier":"53413","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh129002.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh129002.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25232/files/esrh129002.pdf"},"version_id":"441e7148-739e-467a-b2b7-9abc30cc2ad7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"裸子植物","subitem_subject_scheme":"Other"},{"subitem_subject":"MADS-box遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"系統解析","subitem_subject_scheme":"Other"},{"subitem_subject":"ABCモデル","subitem_subject_scheme":"Other"},{"subitem_subject":"生殖器官進化","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Phylogenetic analysis of MADS-box genes in gymnosperms","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Phylogenetic analysis of MADS-box genes in gymnosperms"}]},"item_type_id":"4","owner":"1","path":["2484","2483"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-08-05"},"publish_date":"2013-08-05","publish_status":"0","recid":"25232","relation_version_is_last":true,"title":["Phylogenetic analysis of MADS-box genes in gymnosperms"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:00:18.185536+00:00"}