{"created":"2021-03-01T06:43:10.805912+00:00","id":25237,"links":{},"metadata":{"_buckets":{"deposit":"9b711df5-af03-44f8-b42d-b0a8716b93de"},"_deposit":{"id":"25237","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25237"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025237","sets":["27:2466:2467:2485","9:504:2469:2486"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Eucalyptus robusta 由来のフェニルアラニンアンモニアリアーゼ遺伝子のクローニングと解析"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-02-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"137","bibliographicPageStart":"121","bibliographicVolumeNumber":"128","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Phenylalanine ammonia-lyase (PAL) is the first enzyme of phenylpropanoid pathway. It produces precursors of compounds which play important roles during plant development and defense. Using genome walking technology, a PAL gene designated as ErPAL1 was cloned from Eucalyptus robusta. The full length of ErPAL1 was 3,823 bp. It consists of an intron and two exons encoding a 715 amino-acid polypeptide. Sequence alignment indicated that ErPAL1 shared 70-80% identity with the plant PAL sequences in some previous reports. We also obtained the 5'-flanking sequence and found some putative cis-acting elements such as TATA box, CAAT box, GT1-motif and G-box. ErPAL1 was expressed in Escherichia coli strain BL21 with pEcoli vector. The recombinant protein indicated PAL activity which could catalyze the deamination of L-phenylalanine into trans-cinnamic acid. The optimum temperature and pH for ErPAL1 activity was 50℃ and pH9.0, respectively. The values of Km and kcat for L-phenylalanine were 203 μM and 5.26 s(-1) each. The recombinant protein had similar biochemical properties to other plant PALs.","subitem_description_type":"Abstract"},{"subitem_description":"フェニルアラニンアンモニアリアーゼ(PAL)はフェニルプロパノイド経路の初発酵素である。この酵素反応により, 植物の成長過程や防御作用において重要な役割を担っていると考えられる化合物が生成されている。ユーカリ属樹木Eucalyptus robustaから, ゲノムウオーキングという手法を用いてPALゲノム遺伝子(ErPAL1)ならびにその上流配列をクローニングした。ErPAL1は全長3,823bpで1つのイントロンと2つのエクソン(715アミノ酸をコード)から成り立っており, これまでに報告されている他の植物のPAL遺伝子配列と70-80%の相同性があった。5'フランキング配列にはTATAボックス, CAATボックス, GT1モチーフやGボックスなど多くのシス因子に相当する配列が見られた。pEcoliベクターと大腸菌(BL21)システムを用いて発現させたErPAL1はPAL活性を有していた。さらにErPAL1の酵素学的特性を解析したところ, 最適温度50℃, 最適pH9.0であり, フェニルアラニンに対するKm値203μM, Kcat5.26s(-1)であった。これらの数値は他の植物から得られたPALの値と同様であった。","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"53456","nameIdentifierScheme":"WEKO"}],"names":[{"name":"秋元, 真也"}]},{"nameIdentifiers":[{"nameIdentifier":"53457","nameIdentifierScheme":"WEKO"}],"names":[{"name":"石野, 貴久"}]},{"nameIdentifiers":[{"nameIdentifier":"53458","nameIdentifierScheme":"WEKO"}],"names":[{"name":"寺田, 珠実"}]},{"nameIdentifiers":[{"nameIdentifier":"53459","nameIdentifierScheme":"WEKO"}],"names":[{"name":"鮫島, 正浩"}]},{"nameIdentifiers":[{"nameIdentifier":"53460","nameIdentifierScheme":"WEKO"}],"names":[{"name":"鴨田, 重裕"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The University of Tokyo Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Arboricultural Research Institute, The University of Tokyo Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"東京大学大学院農学生命科学研究科附属演習林樹芸研究所"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Akimoto, Shinya"}],"nameIdentifiers":[{"nameIdentifier":"53451","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishino, Takahisa"}],"nameIdentifiers":[{"nameIdentifier":"53452","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Terada, Tamami"}],"nameIdentifiers":[{"nameIdentifier":"53453","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Samejima, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"53454","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamoda, Shigehiro"}],"nameIdentifiers":[{"nameIdentifier":"53455","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh128004.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh128004.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25237/files/esrh128004.pdf"},"version_id":"5494f248-b7ec-4699-ab24-8565fff8bf85"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Eucalyptus robusta","subitem_subject_scheme":"Other"},{"subitem_subject":"expression","subitem_subject_scheme":"Other"},{"subitem_subject":"molecular cloning","subitem_subject_scheme":"Other"},{"subitem_subject":"phenylalanine ammonia-lyase (PAL)","subitem_subject_scheme":"Other"},{"subitem_subject":"発現","subitem_subject_scheme":"Other"},{"subitem_subject":"クローニング","subitem_subject_scheme":"Other"},{"subitem_subject":"フェニルアラニンアンモニアリアーゼ(PAL)","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A phenylalanine ammonia-lyase gene (ErPAL1) from Eucalyptus robusta : molecular cloning, expression and characterization : Running title : Molecular cloning of ErPAL1 from cultured eucalyptus cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A phenylalanine ammonia-lyase gene (ErPAL1) from Eucalyptus robusta : molecular cloning, expression and characterization : Running title : Molecular cloning of ErPAL1 from cultured eucalyptus cells"}]},"item_type_id":"4","owner":"1","path":["2486","2485"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-03-01"},"publish_date":"2013-03-01","publish_status":"0","recid":"25237","relation_version_is_last":true,"title":["A phenylalanine ammonia-lyase gene (ErPAL1) from Eucalyptus robusta : molecular cloning, expression and characterization : Running title : Molecular cloning of ErPAL1 from cultured eucalyptus cells"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:34.326948+00:00"}