{"created":"2021-03-01T06:43:13.003736+00:00","id":25271,"links":{},"metadata":{"_buckets":{"deposit":"f78eb3de-d667-4545-bd75-40c0cb239294"},"_deposit":{"id":"25271","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25271"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025271","sets":["27:2466:2467:2501","9:504:2469:2502"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Ability of Taxoids Production in Japanese Taxaceae Plants"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"52","bibliographicPageStart":"45","bibliographicVolumeNumber":"120","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"キャラボク，カヤにおけるタキソイド生成能の有無を，タキソイド生成関連酵素をコードする遺伝子配列をもつかどうかという観点からイチイと比較検討した。この3 種類の植物の葉よりDNA を抽出後，塩基配列既知のTaxus × media，Taxus brevifolia から作成した7 種類の酵素のプライマーを用いて，PCR を行い，そのPCR 産物の塩基配列を読んで相同性を調べた。また，タキソイド生成に関するキーエンザイムと考えられるTXS においては，ドットブロットハイブリダイゼーションも行った。その結果，キャラボクでは7 種類の酵素すべてで配列が読め，高い相同性を示したので，他のイチイ属樹木と同様にタキソイド生成能を有していると推測された。カヤではGGPPS,TαH，BAPTの3 種類の酵素遺伝子が確認でき，TXSに関してもドットブロットハイブリダイゼーションでスポットが確認された。","subitem_description_type":"Abstract"},{"subitem_description":"We researched the gene sequences of paclitaxel biosynthetic pathway enzymes in Taxus cuspidata var. nana and Torreya nucifera to discover whether they have the ability of taxoid production, using Taxus cuspidata as a comparison. Genomic DNA was extracted from the needles of the three species. Sequences of seven paclitaxel biosynthetic pathway genes were amplified from genomic DNA by PCR with primer pairs based on the sequences of Taxus×media and Taxus brevifolia and these were analyzed. We also examined the presence of TXS, thought to be a key enzyme for taxoid production, by dot blot hybridization. As a result, we were able to obtain the sequences of seven paclitaxel biosynthetic pathway genes from Taxus cuspidata var. nana and from this we assumed that Taxus cuspidata var. nana has the ability of taxoid production. The three sequences of GGPPS, TαH and BAPT were observed in Torreya nucifera. In addition, TXS hybridization spots were observed. These indicated that Torreya nucifera probably has the sequence of TXS.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"53670","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ISHINO, Takahisa"}]},{"nameIdentifiers":[{"nameIdentifier":"53671","nameIdentifierScheme":"WEKO"}],"names":[{"name":"AIZAWA, Eishi"}]},{"nameIdentifiers":[{"nameIdentifier":"53672","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TERADA, Tamami"}]},{"nameIdentifiers":[{"nameIdentifier":"53673","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SAMEJIMA, Masahiro"}]},{"nameIdentifiers":[{"nameIdentifier":"53674","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMODA, Shigehiro"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"独立行政法人 国際協力機構"},{"subitem_text_value":"東京大学大学院農学生命科学研究科附属演習林樹芸研究所"},{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Japan International Cooperation Agency"},{"subitem_text_value":"Arboricultural Research Institute, University Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石野, 貴久"}],"nameIdentifiers":[{"nameIdentifier":"53665","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"会沢, 栄志"}],"nameIdentifiers":[{"nameIdentifier":"53666","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田, 珠実"}],"nameIdentifiers":[{"nameIdentifier":"53667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鮫島, 正浩"}],"nameIdentifiers":[{"nameIdentifier":"53668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鴨田, 重裕"}],"nameIdentifiers":[{"nameIdentifier":"53669","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"ersh120005.pdf","filesize":[{"value":"811.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ersh120005.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25271/files/ersh120005.pdf"},"version_id":"21329d30-511e-4313-a381-8d643fcdee84"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"イチイ科植物","subitem_subject_scheme":"Other"},{"subitem_subject":"タキソイド","subitem_subject_scheme":"Other"},{"subitem_subject":"PCRクローニング","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA塩基配列解析","subitem_subject_scheme":"Other"},{"subitem_subject":"ドットブロットハイブリダイゼーション","subitem_subject_scheme":"Other"},{"subitem_subject":"taxaceae","subitem_subject_scheme":"Other"},{"subitem_subject":"taxoid","subitem_subject_scheme":"Other"},{"subitem_subject":"dot blot hybridization","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR cloninig","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA sequencing","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"日本産イチイ科植物におけるタキソイド生成能","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"日本産イチイ科植物におけるタキソイド生成能"}]},"item_type_id":"4","owner":"1","path":["2502","2501"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-17"},"publish_date":"2012-07-17","publish_status":"0","recid":"25271","relation_version_is_last":true,"title":["日本産イチイ科植物におけるタキソイド生成能"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:00:27.302598+00:00"}