{"created":"2021-03-01T06:43:14.228671+00:00","id":25290,"links":{},"metadata":{"_buckets":{"deposit":"80cce356-5b82-4ebe-ba28-935b9387fa9d"},"_deposit":{"id":"25290","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25290"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025290","sets":["27:2466:2467:2509","9:504:2469:2510"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Relation of Cell Growth and Phytosterols in Cell Cultures of Ginkgo biloba"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"265","bibliographicPageStart":"253","bibliographicVolumeNumber":"116","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"イチョウ培養細胞中に含まれる植物ステロール類を分析し，その生合成経路と役割を調べた。イチョウからはβ- シトステロールのほかにカンペステロールとスチグマステロールが検出された。β- シトステロールは細胞増殖とともに生成されることがわかった。ステロールの生合成阻害剤のひとつであるアンシミドールを投与すると，細胞増殖が阻害され，細胞中のステロール量も減少した。アンシミドール処理細胞にβ- シトステロールを添加すると，培地成分の取り込みでは回復傾向が見られたが細胞増殖の回復はほとんど確認できなかった。メバロン酸生成の特異的阻害剤であるコンパクチンを投与すると細胞増殖が阻害され，細胞中のステロール量も減少した。イチョウにおいてステロールはメバロン酸経路で生成されると考えられた。また，コンパクチン処理細胞にメバロン酸を添加すると細胞増殖は回復する傾向を見せたが，β- シトステロールを添加した際にはむしろ阻害された。これらのことからイチョウ培養細胞において，ステロールは細胞増殖における重要な因子の一つではあることがわかった。しかしステロールのみが細胞増殖に関わっているのではなく他のメバロン酸代謝物の影響も考え合わせなくてはいけないことがわかった。","subitem_description_type":"Abstract"},{"subitem_description":"The biosynthesis pathway and roles of phytosterols in suspension cell cultures of Ginkgo biloba were investigated. Three kinds of sterols (campesterol, stigmasterol and β-sitosterol) were detected in Ginkgo cells and β-sitosterol might be produced by cell growth. Treatment with ancymidol, one of the sterol synthesis inhibitors, reduced cell growth and sterol contents. Addition ofβ-sitosterol restored the incorporation of medium components, but not cell growth. Treatment with compactin, a specific inhibitor of mevalonic acid synthesis, reduced both cell growth and sterol amount. Addition of mevalonic acid to compactin-treated cells could induce cell growth, but the addition of β-sitosterol caused a decrease in cell growth. These results suggest that sterols might be one of the necessary factors for cell growth, while the other metabolites via the mevalonic acid pathway should be considered.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"53801","nameIdentifierScheme":"WEKO"}],"names":[{"name":"INOUE, Hiroki"}]},{"nameIdentifiers":[{"nameIdentifier":"53802","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KANEYUKI, Tamijiro"}]},{"nameIdentifiers":[{"nameIdentifier":"53803","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TERADA, Tamami"}]},{"nameIdentifiers":[{"nameIdentifier":"53804","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMODA, Shigehiro"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"東京大学農学部附属演習林樹芸研究所"},{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Arboricultural Research Institute, University Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"井上, 広喜"}],"nameIdentifiers":[{"nameIdentifier":"53797","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"兼行, 民治郎"}],"nameIdentifiers":[{"nameIdentifier":"53798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田, 珠実"}],"nameIdentifiers":[{"nameIdentifier":"53799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鴨田, 重裕"}],"nameIdentifiers":[{"nameIdentifier":"53800","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh116007.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh116007.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25290/files/esrh116007.pdf"},"version_id":"ce089521-2bfa-42d9-a08f-4f215c5a699d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"イチョウ培養細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ステロール","subitem_subject_scheme":"Other"},{"subitem_subject":"生合成阻害剤","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞増殖","subitem_subject_scheme":"Other"},{"subitem_subject":"メバロン酸","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell cultures of Ginkgo biloba","subitem_subject_scheme":"Other"},{"subitem_subject":"Sterol","subitem_subject_scheme":"Other"},{"subitem_subject":"Biosynthesis inhibitor","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell growth","subitem_subject_scheme":"Other"},{"subitem_subject":"Mevalonic acid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"イチョウ細胞培養系におけるステロールと細胞増殖","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"イチョウ細胞培養系におけるステロールと細胞増殖"}]},"item_type_id":"4","owner":"1","path":["2510","2509"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-17"},"publish_date":"2012-07-17","publish_status":"0","recid":"25290","relation_version_is_last":true,"title":["イチョウ細胞培養系におけるステロールと細胞増殖"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:36.259482+00:00"}