{"created":"2021-03-01T06:43:14.550927+00:00","id":25295,"links":{},"metadata":{"_buckets":{"deposit":"b6b4c80b-4c09-4d9a-bb97-24f02c834ba4"},"_deposit":{"id":"25295","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25295"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025295","sets":["27:2466:2467:2511","9:504:2469:2512"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Analysis of the genes of Lignostilbenedioxygenase isozymes"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"72","bibliographicPageStart":"65","bibliographicVolumeNumber":"115","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"スチルベンは有名なファイトアレキシンの一つである。この研究ではそのスチルベンを分解する能力を持つ細菌Sphingomonas paucimobilis TMY1009 を取り上げる。スチルベン型ファイトアレキシンとそれを分解する菌の産生する酵素リグノスチルベンジオキシゲナーゼの関係は，ファイトアレキシンを生産する植物とそれを攻撃する微生物との攻防を探るモデルケースである。リグノスチルベンジオキシゲナーゼはα，β，γ サブユニットからなる二量体酵素でαα，αβ，ββ，γγ の4 種のアイソザイムが確認されている。第三のγ サブユニットをペプチダーゼで分解し，分解産物のN末端アミノ酸配列情報を得，それを基に未知のγ サブユニット遺伝子を検出するプライマーセットを開発した。それにより既知のlsdA,Bと異なるlsd遺伝子の検出が確認された。それはγサブユニット（lsdC）遺伝子と思われた。","subitem_description_type":"Abstract"},{"subitem_description":"We wished to gain a better understanding of the interaction between plants and the microorganisms around plants through chemical interaction. Our study was designed to understand the offensive and defensive battle between plants armed with stilbenoid and microorganisms armed with stilbenoid detoxication enzyme. Stilbenoid is one of the most wellknown phytoalexins. We focused on Sphingomonas paucimobilis TMY1009 that produced four lignostilbenedioxygenase (LSD) isozymes, degrading stilbenoid type phytoalexin. LSD isozymes I, II, III, and IV were composed of αα, αβ, ββ and γγ subunits, respectively, and showed different substrate specificities. The genes of α and β subunits of LSDs have already been cloned and sequenced.The peptides of γ subunits digested by peptidase were analyzed for their N terminal amino acids. The deduced primers for the γ subunit made it possible to detect an unknown lsd gene that was thought to be the gene of the γ subunit (lsdC).","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"53846","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMODA, Shigehiro"}]},{"nameIdentifiers":[{"nameIdentifier":"53847","nameIdentifierScheme":"WEKO"}],"names":[{"name":"INOUE, Hiroki"}]},{"nameIdentifiers":[{"nameIdentifier":"53848","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TERADA, Tamami"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院農学生命科学研究科附属演習林樹芸研究所"},{"subitem_text_value":"理化学研究所植物科学研究センター"},{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"Arboricultural Research Institute, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"RIKEN Plant Science Center"},{"subitem_text_value":"Department of Biomaterial Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鴨田, 重裕"}],"nameIdentifiers":[{"nameIdentifier":"53843","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井上, 広喜"}],"nameIdentifiers":[{"nameIdentifier":"53844","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田, 珠実"}],"nameIdentifiers":[{"nameIdentifier":"53845","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh115004.pdf","filesize":[{"value":"536.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh115004.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25295/files/esrh115004.pdf"},"version_id":"44cba416-994d-46d8-8b53-0261fb0973e9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"リグノスチルベン","subitem_subject_scheme":"Other"},{"subitem_subject":"リグノスチルベンジオキシゲナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"サブユニット","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"検出プライマー","subitem_subject_scheme":"Other"},{"subitem_subject":"Lignostilbene","subitem_subject_scheme":"Other"},{"subitem_subject":"lignostilbenedioxygenase","subitem_subject_scheme":"Other"},{"subitem_subject":"subunit","subitem_subject_scheme":"Other"},{"subitem_subject":"gene","subitem_subject_scheme":"Other"},{"subitem_subject":"detection primers","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"リグノスチルベンジオキシゲナーゼアイソザイム遺伝子群の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"リグノスチルベンジオキシゲナーゼアイソザイム遺伝子群の解析"}]},"item_type_id":"4","owner":"1","path":["2512","2511"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-17"},"publish_date":"2012-07-17","publish_status":"0","recid":"25295","relation_version_is_last":true,"title":["リグノスチルベンジオキシゲナーゼアイソザイム遺伝子群の解析"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:36.344120+00:00"}