{"created":"2021-03-01T06:43:14.750394+00:00","id":25298,"links":{},"metadata":{"_buckets":{"deposit":"5203d8a0-afab-448a-bf63-160f3a613fd5"},"_deposit":{"id":"25298","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25298"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025298","sets":["27:2466:2467:2513","9:504:2469:2514"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Mechanism of Secondary Metabolites Production in Poplar Suspension Cell Cultures (2) : Effects of Cytokinins on Secondary Metabolites Production"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographicVolumeNumber":"114","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ポプラ（× Populus deltoides cv. I-72/51）懸濁培養細胞に，エリシターとしてペクチナーゼを添加するといくつかの二次代謝物の生成が見られるので，その生成機構について検討した。とくにガスクロマトグラム上で顕著なピークを示す二次代謝物Xに着目した。傷害誘導性遺伝子発現に関わっているといわれているジャスモン酸類とシステミンをポプラ培養細胞に投与した。ジャスモン酸類の添加ではペクチナーゼ処理の30%程度の二次代謝物Xの生成が見られたが，システミンの添加では全く二次代謝物Xは生成されなかった。次に傷害応答物質を誘導するといわれているカイネチンを添加してみた。カイネチンのみを添加した場合，特異的二次代謝物を生成することはなかった。ペクチナーゼとともにカイネチン（120 μM以上）を投与したところ二次代謝物Xの生成が著しく抑制されることがわかった。そこで他のアミノプリン系サイトカイニン（ベンジルアミノプリン，ゼアチン）とウレア系サイトカイニン（チジアズロン）についてカイネチンと同様の実験を行ったところ，いずれの場合もペクチナーゼとともに添加したときに二次代謝物Xの生成を抑制することが示された。ペクチナーゼに代わるシグナル伝達物質を見つけることはできなかったが，ポプラ培養細胞の二次代謝物生成機構にサイトカイニンが関与していることが示唆された。サイトカイニンの作用機構については現在検討中である。","subitem_description_type":"Abstract"},{"subitem_description":"We investigated the mechanism of some secondary metabolites production elicited by pectinase in poplar (× Populus deltoides cv. I-72/51) suspension cell cultures using jasmonates, systemin, and cytokinins, known as signal factors of plants. Treatment of jasmonates caused the production of secondary metabolites, and the amount of metabolites X induced by jasmonates reached 30% of the level observed with a pectinase treatment. However addition of systemin could not induce metabolite production. Kinetin added alone had no effect on the production of the metabolites, whereas added in large quantities (more than 120 μM) with pectinase it repressed the production of the metabolites. Other cytokinins, 6-benzylaminopurine, zeatin (aminopurine type) thidiazuron (urea type), also caused the inhibition of metabolites X production. These results suggest that cytokinins might be negatively related with the pectinase-induced production of secondary metabolites.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"53873","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SATO, Mari"}]},{"nameIdentifiers":[{"nameIdentifier":"53874","nameIdentifierScheme":"WEKO"}],"names":[{"name":"INOUE, Hiroki"}]},{"nameIdentifiers":[{"nameIdentifier":"53875","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMODA, Shigehiro"}]},{"nameIdentifiers":[{"nameIdentifier":"53876","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TERADA, Tamami"}]},{"nameIdentifiers":[{"nameIdentifier":"53877","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SABURI, Yoshimasa"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"東京大学大学院農学生命科学研究科附属演習林樹芸研究所"},{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Arboricultural Research Institute, University Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 万里"}],"nameIdentifiers":[{"nameIdentifier":"53868","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井上, 広喜"}],"nameIdentifiers":[{"nameIdentifier":"53869","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鴨田, 重裕"}],"nameIdentifiers":[{"nameIdentifier":"53870","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田, 珠美"}],"nameIdentifiers":[{"nameIdentifier":"53871","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"左分, 義正"}],"nameIdentifiers":[{"nameIdentifier":"53872","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh114001.pdf","filesize":[{"value":"792.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh114001.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25298/files/esrh114001.pdf"},"version_id":"b359615c-60bb-496a-8724-011345a4ff2b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ポプラ培養細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"二次代謝物","subitem_subject_scheme":"Other"},{"subitem_subject":"ペクチナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"ジャスモン酸","subitem_subject_scheme":"Other"},{"subitem_subject":"サイトカイニン","subitem_subject_scheme":"Other"},{"subitem_subject":"Poplar cell cultures","subitem_subject_scheme":"Other"},{"subitem_subject":"Secondary metabolites","subitem_subject_scheme":"Other"},{"subitem_subject":"Pectinase","subitem_subject_scheme":"Other"},{"subitem_subject":"Jasmonates","subitem_subject_scheme":"Other"},{"subitem_subject":"Cytokinin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ポプラ懸濁培養細胞における二次代謝物生成機構（その2） : サイトカイニンの影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ポプラ懸濁培養細胞における二次代謝物生成機構（その2） : サイトカイニンの影響"}]},"item_type_id":"4","owner":"1","path":["2514","2513"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-17"},"publish_date":"2012-07-17","publish_status":"0","recid":"25298","relation_version_is_last":true,"title":["ポプラ懸濁培養細胞における二次代謝物生成機構（その2） : サイトカイニンの影響"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:38.353854+00:00"}