{"created":"2021-03-01T06:43:16.714021+00:00","id":25328,"links":{},"metadata":{"_buckets":{"deposit":"6dfefcab-5691-43b5-b581-c465a57f2bc0"},"_deposit":{"id":"25328","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25328"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025328","sets":["27:2466:2467:2523","9:504:2469:2524"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"PCR Cloning and DNA Sequencing of Chalcone Synthase Gene of Picea jezoensis"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographicVolumeNumber":"109","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"2年生のエゾマツの新葉からゲノムDNAを抽出し，Picea marianaのカルコン合成酵素の塩基配列を基に設計したプライマーを用いてPCR増幅したDNA断片をクローニングした。48個のうち，PCRスクリーニングで陽性と思われるクローンを33個得ることができた。4クローンについてはさらに塩基配列を解析し，N末端とC末端のprimer部分を除く全塩基配列を決定した。活性に必須と考えられているCys169や，loopII～IVの存在が確認されたことから，これらの遺伝子はカルコン合成酵素をコードするものと思われた。","subitem_description_type":"Abstract"},{"subitem_description":"Genomic DNA was extracted from new needles of 2-year-old Picea jezoensis. Sequences of chalcone synthase (CHS) genes were amplified from the genomic DNA by PCR with primer pairs designed based on the CHS sequence of Picea mariana. The products of PCR were cloned into a plasmid vector. Forty-eight clones were tested for PCR screening, and 33 clones were obtained. The sequences about 4 of them were analyzed. All sequences, except for the part of primer used for amplification of CHS genes, were determined. These were considerd to be CHS genes by comparison with that of P. mariana. Because all of them have Cys169, loopII, loopIII and loopIV that is required for active enzymes, they do not seem to be pseudogene.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54058","nameIdentifierScheme":"WEKO"}],"names":[{"name":"MATSUMOTO, Maki"}]},{"nameIdentifiers":[{"nameIdentifier":"54059","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KANEYUKI, Tamijiro"}]},{"nameIdentifiers":[{"nameIdentifier":"54060","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TERADA, Tamami"}]},{"nameIdentifiers":[{"nameIdentifier":"54061","nameIdentifierScheme":"WEKO"}],"names":[{"name":"INOUE, Hiroki"}]},{"nameIdentifiers":[{"nameIdentifier":"54062","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMODA, Shigehiro"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院農学生命科学研究科生圏システム学専攻"},{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"理化学研究所植物科学研究センター"},{"subitem_text_value":"東京大学大学院農学生命科学研究科附属北海道演習林"},{"subitem_text_value":"Department of Ecosystem Studies, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Laboratory for Adaptation and Resistance, RIKEN Plant Science Center"},{"subitem_text_value":"The University Forest in Hokkaido, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松本, 麻紀"}],"nameIdentifiers":[{"nameIdentifier":"54053","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"兼行, 民治郎"}],"nameIdentifiers":[{"nameIdentifier":"54054","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田, 珠実"}],"nameIdentifiers":[{"nameIdentifier":"54055","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井上, 広喜"}],"nameIdentifiers":[{"nameIdentifier":"54056","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鴨田, 重裕"}],"nameIdentifiers":[{"nameIdentifier":"54057","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh109001.pdf","filesize":[{"value":"444.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh109001.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25328/files/esrh109001.pdf"},"version_id":"2b95fd5e-3b33-4371-b0ec-d3d5e38693c5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"エゾマツ","subitem_subject_scheme":"Other"},{"subitem_subject":"カルコン合成酵素遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"PCRクローニング","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA塩基配列解析","subitem_subject_scheme":"Other"},{"subitem_subject":"Picea jezoensis","subitem_subject_scheme":"Other"},{"subitem_subject":"chalcone synthase genes","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR cloning","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA sequencing","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"エゾマツのカルコン合成酵素遺伝子のPCRクローニングと塩基配列","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"エゾマツのカルコン合成酵素遺伝子のPCRクローニングと塩基配列"}]},"item_type_id":"4","owner":"1","path":["2524","2523"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-07-17"},"publish_date":"2012-07-17","publish_status":"0","recid":"25328","relation_version_is_last":true,"title":["エゾマツのカルコン合成酵素遺伝子のPCRクローニングと塩基配列"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:00:48.062523+00:00"}