{"created":"2021-03-01T06:43:19.553747+00:00","id":25372,"links":{},"metadata":{"_buckets":{"deposit":"05ac2392-5ca0-4a0d-8268-a6e12a3780a7"},"_deposit":{"id":"25372","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25372"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025372","sets":["27:2466:2467:2537","9:504:2469:2538"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Popu Iuseuphratica Oiiv. の組織培養系の確立"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"92","bibliographicPageStart":"87","bibliographicVolumeNumber":"102","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Populus euphraticaの標準的な培養系を確立した。温室内における水耕栽培によって育てたP. euphraticaの苗からえき芽のついた茎軸の小片を切り分け，表面殺菌後BAPを添加したMSあるいは1/2MS培地に植え付けた。その結果，1/2MSにBAPを0.25mg/l単独で，あるいは0.05mg/lのNAAとともに添加した培地が，シュートの伸長とマルチプルシュートの形成に最も適していた。0.8mg/lのBAPを添加した1/2MS培地で一旦継代培養した後，継代培養に適した培地条件を検討した。その結果，0.2mg/lのBAPを添加した培地でもっともガラス化するシュートの割合が少なく健全なシュートが得られた。BAPを含まない培地ではNAAの添加の有無に関わらずシュートからの発根が認められた。NAAを0.01mg/l添加した場合には発根率がもっとも高く90％に達した。得られた植物体は，ほぼ100％順化が可能であり，温室内に置いて3ヶ月後には50～80cmの苗木に育った。","subitem_description_type":"Abstract"},{"subitem_description":"A standard tissue culture system of Populus euphratica was established. Stem segments with axillary buds were excised from seedlings grown hydroponically in a greenhouse. They were surface-sterilized and cultured on MS or 1/2MS medium supplemented with various concentrations of BAP and NAA. Among the tested media, 1/2MS with 0.25mg/l of BAP alone or in combination with 0.05mg/l of NAA were optimal for shoot elongation and multiple shoot formation. After subculture on 1/2MS with 0.8mg/l of BAP, the optimal BAP and NAA concentration was surveyed for rapid clonal propagation. Shoots grew most healthily and the rate of vitrification was the lowest on the 1/2MS medium supplemented with 0.2mg/l of BAP. Rooting occurred on media without BAP irrespective of NAA concentration. The ratio of rooted shoots increased up to the highest of 90% at the concentration of 0.01mg/l NAA after three weeks of culture. Almost all plantlets were successfully acclimatized and grew into 50-80cm tall saplings in a greenhouse three months after transplanting to the potting soil.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54345","nameIdentifierScheme":"WEKO"}],"names":[{"name":"渡辺, 信"}]},{"nameIdentifiers":[{"nameIdentifier":"54346","nameIdentifierScheme":"WEKO"}],"names":[{"name":"小島, 克己"}]},{"nameIdentifiers":[{"nameIdentifier":"54347","nameIdentifierScheme":"WEKO"}],"names":[{"name":"井出, 雄二"}]},{"nameIdentifiers":[{"nameIdentifier":"54348","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐々木, 恵彦"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Forest Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Asian Natural Environmental Science Center, The University of Tokyo"},{"subitem_text_value":"University Forests in Chiba, Faculty of Agriculture, The University of Tokyo"},{"subitem_text_value":"College of Bioresource Sciences, Nihon University"},{"subitem_text_value":"東京大学大学院農学生命科学研究科"},{"subitem_text_value":"東京大学アジア生物資源環境研究センター"},{"subitem_text_value":"東京大学農学部付属千葉演習林"},{"subitem_text_value":"日本大学生物資源科学部"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"WATANABE, Shin"}],"nameIdentifiers":[{"nameIdentifier":"54341","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KOJIMA, Katsumi"}],"nameIdentifiers":[{"nameIdentifier":"54342","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IDE, Yuji"}],"nameIdentifiers":[{"nameIdentifier":"54343","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SASAKI, Satohiko"}],"nameIdentifiers":[{"nameIdentifier":"54344","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh102003.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh102003.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25372/files/esrh102003.pdf"},"version_id":"a7e14d96-85a0-4cfd-b128-b97deae8588b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Populus euphratica","subitem_subject_scheme":"Other"},{"subitem_subject":"組織培養","subitem_subject_scheme":"Other"},{"subitem_subject":"植物体再生","subitem_subject_scheme":"Other"},{"subitem_subject":"えき芽","subitem_subject_scheme":"Other"},{"subitem_subject":"継代培養","subitem_subject_scheme":"Other"},{"subitem_subject":"Populus euphratica","subitem_subject_scheme":"Other"},{"subitem_subject":"tissue culture","subitem_subject_scheme":"Other"},{"subitem_subject":"plant regeneration","subitem_subject_scheme":"Other"},{"subitem_subject":"axillary buds","subitem_subject_scheme":"Other"},{"subitem_subject":"subculture","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Establishment of a Tissue Culture System of Populus euphratica Oliv","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Establishment of a Tissue Culture System of Populus euphratica Oliv"}]},"item_type_id":"4","owner":"1","path":["2538","2537"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25372","relation_version_is_last":true,"title":["Establishment of a Tissue Culture System of Populus euphratica Oliv"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:39.146637+00:00"}