{"created":"2021-03-01T06:43:19.683876+00:00","id":25374,"links":{},"metadata":{"_buckets":{"deposit":"fb7fcabe-56af-4bf7-9a82-d685d26c0a82"},"_deposit":{"id":"25374","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25374"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025374","sets":["27:2466:2467:2537","9:504:2469:2538"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Invitro におけるモミ実生からの植物体再生"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"111","bibliographicPageStart":"103","bibliographicVolumeNumber":"102","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"モミ（Abies firma）について，幼根を除去した芽生えの発根および下胚軸片からの不定芽形成による植物体再生法を確立した。幼根を除いた芽生えの針葉の形成にはSH培地が適していた。特に，1.0mg/lのBAPを添加したSH培地で新たな針葉形成が最大となった。新たな針葉の展開した芽生えは1,000ppmのNAA水溶液に基部を1分間浸漬した後，0.3％の活性炭を添加したホルモンを含まない1/2SH培地に移植したところ，発根して植物体が再生した。発根率は63.6％であった。培養5週間後に，BAP1.0mg/lとNAA0.3mg/lを添加したSH培地上で培養した下胚軸片から，不定芽が形成された。これらをホルモンを含まない1/2SH培地に移すシュートの形成が見られ，伸長したシュートは幼根を幼根を除いた芽生えの場合と同じ方法で発根させ植物体を再生させることが出来た。","subitem_description_type":"Abstract"},{"subitem_description":"Two successful plant regeneration procedures for Abies firma from seedlings from which radicles were removed and from excised hypocotyl segments through adventitious bud formation were established. SH medium was found to be adaptable to needle development of de-rooted seedlings. They developed the maximum number of new needles on SH medium containing 1.0mg/l BAP and rooted on hormone-free 1/2SH medium containing 0.3% activated charcoal following immersion in 1,000ppm NAA solution for 1 min. The frequency of rooted explants was 63.6%. Adventitious buds were induced on excised hypocotyls on SH medium supplemented with 1.0mg/l BAP and 0.3mg/l NAA after 5 weeks culture. Elongation of adventitious buds was promoted on hormone-free 1/2SH medium. Regenerated plantlets were successfully obtained by the procedure used in the rooting of de-rooted seedlings as described above.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54356","nameIdentifierScheme":"WEKO"}],"names":[{"name":"ワーリオ, 禄敏"}]},{"nameIdentifiers":[{"nameIdentifier":"54357","nameIdentifierScheme":"WEKO"}],"names":[{"name":"田中, 恵"}]},{"nameIdentifiers":[{"nameIdentifier":"54358","nameIdentifierScheme":"WEKO"}],"names":[{"name":"井出, 雄二"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Research Division of the University Forests, Faculty of Agriculture, The University of Tokyo"},{"subitem_text_value":"東京大学大学院農学生命科学研究科森林科学専攻"},{"subitem_text_value":"東京大学農学部附属演習林研究部"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"VAARIO, L-M."}],"nameIdentifiers":[{"nameIdentifier":"54353","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TANAKA, Megumi"}],"nameIdentifiers":[{"nameIdentifier":"54354","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IDE, Yuji"}],"nameIdentifiers":[{"nameIdentifier":"54355","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh102005.pdf","filesize":[{"value":"968.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh102005.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25374/files/esrh102005.pdf"},"version_id":"fff23961-2dba-4f1d-9f87-3f9bfde9d4f4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"モミ（Abies firma）","subitem_subject_scheme":"Other"},{"subitem_subject":"植物体再生","subitem_subject_scheme":"Other"},{"subitem_subject":"組織培養","subitem_subject_scheme":"Other"},{"subitem_subject":"下胚軸","subitem_subject_scheme":"Other"},{"subitem_subject":"不定芽","subitem_subject_scheme":"Other"},{"subitem_subject":"Abies firma","subitem_subject_scheme":"Other"},{"subitem_subject":"plant regeneration","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro culture","subitem_subject_scheme":"Other"},{"subitem_subject":"hypocotyl","subitem_subject_scheme":"Other"},{"subitem_subject":"adventitious bud","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In vitro Plantlet Regeneration of Abies firma from Germinated Seedlings","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vitro Plantlet Regeneration of Abies firma from Germinated Seedlings"}]},"item_type_id":"4","owner":"1","path":["2538","2537"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25374","relation_version_is_last":true,"title":["In vitro Plantlet Regeneration of Abies firma from Germinated Seedlings"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:59:55.823183+00:00"}