{"created":"2021-03-01T06:43:19.876221+00:00","id":25377,"links":{},"metadata":{"_buckets":{"deposit":"b65b8e68-4090-48b8-9a30-bffc1a4b254c"},"_deposit":{"id":"25377","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25377"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025377","sets":["27:2466:2467:2537","9:504:2469:2538"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"NaCI を含む培地における Populuse uphratica，Populus  albacv.Pyramidalis, Populus maximowiczii× Populus plantierensis の培養細胞の成長"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"150","bibliographicPageStart":"143","bibliographicVolumeNumber":"102","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Populus euphratica, Populus alba cv. Pyramidalis, Populus maximowiczii×Populus plantierensisの3種のポプラの培養細胞について，培地へのNaClの添加量を徐々に増加させた場合の耐性を評価した。NaClを添加した最初の培養では，P. euphratica, P. maximowiczii×P. plantierensisの細胞は，200mMのNaCl添加では成長が著しく抑制された。また，P. alba cv. Pyramidalisの細胞では150mMのNaCl添加で成長が抑制された。しかし，培養を繰り返すうちに細胞はNaClに対する適応性を発達させ，6回目の継代培養終了時には，P. euphraticaの細胞は200mM, P. alba cv. Pyramidalisの細胞は150mMのNaCl添加培地でも良好な成長が可能になった。しかし，P. maximowiczii×P. plantierensisの細胞は，100mMまででしか良好な成長を示さなくなった。高NaCl条件下において種ごとの細胞成長に違いが見られたが，すべての種において9回目の継代培養において200mMのNaCl条件下での細胞の生存が確認された。また，NaCl200mMで培養された細胞は，5回から6回目の継代培養後でもカルスの再生能力を有していた。特に，P. euphraticaではNaCl250mMの条件で培養した細胞からもカルスが再生した。","subitem_description_type":"Abstract"},{"subitem_description":"Tolerance for gradually increasing NaCl stress was evaluated for the suspension cultured cells of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii×Populus plantierensis. In the first culture in a medium containing more than 200 mM NaCl, growth of P. euphratica and P. maximowiczii×P. plantierensis cells was remarkably suppressed. Growth of P. alba cv. Pyramidalis cells was suppressed in a medium containing more than 150mM NaCl. During the subculture, P. euphratica and in P. alba cv. Pyramidalis cells developed their adaptability for NaCl stress. At the end of the sixth subculture, P. euphratica could grow well in a medium containing 200mM NaCl and P. alba cv. Pyramidalis could grow well in a medium containing 150mM NaCl. On the other hand, P. maximowiczii×P. plantierensis reduced its adaptability for NaCl concentration to 100mM. Although there were differences between species in their ability of cell growth under high NaCl concentration, cells could survive in 200mM NaCl after the ninth subculture in every species. Particularly P. euphratica could divide even in a medium containing 250mM NaCl. Such surviving cells still have the ability for callus regeneration after the fifth or sixth subculture.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54378","nameIdentifierScheme":"WEKO"}],"names":[{"name":"沈, 海龍"}]},{"nameIdentifiers":[{"nameIdentifier":"54379","nameIdentifierScheme":"WEKO"}],"names":[{"name":"渡辺, 信"}]},{"nameIdentifiers":[{"nameIdentifier":"54380","nameIdentifierScheme":"WEKO"}],"names":[{"name":"井出, 雄二"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Research Division of the University Forests, Faculty of Agriculture, The University of Tokyo"},{"subitem_text_value":"Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Faculty of Forest Resources and Environment, Northeast Forestry University"},{"subitem_text_value":"東京大学農学部附属演習林研究部"},{"subitem_text_value":"東京大学大学院農学生命科学研究科森林科学専攻"},{"subitem_text_value":"中国東北林業大学森林資源与環境学院"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SHEN, Hailong"}],"nameIdentifiers":[{"nameIdentifier":"54375","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"WATANABE, Shin"}],"nameIdentifiers":[{"nameIdentifier":"54376","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IDE, Yuji"}],"nameIdentifiers":[{"nameIdentifier":"54377","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh102008.pdf","filesize":[{"value":"563.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh102008.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25377/files/esrh102008.pdf"},"version_id":"bb5c6fe0-4d02-407e-8b08-bce08b86a326"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"NaClストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"培養細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"カルス再生","subitem_subject_scheme":"Other"},{"subitem_subject":"Populus euphratica","subitem_subject_scheme":"Other"},{"subitem_subject":"Populus alba cv. Pyramidalis","subitem_subject_scheme":"Other"},{"subitem_subject":"Populus maximowiczii×Populus plantierensis","subitem_subject_scheme":"Other"},{"subitem_subject":"NaCl stress","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell suspension culture","subitem_subject_scheme":"Other"},{"subitem_subject":"Callus regeneration","subitem_subject_scheme":"Other"},{"subitem_subject":"Populus euphratica","subitem_subject_scheme":"Other"},{"subitem_subject":"Populus alba cv. Pyramidalis","subitem_subject_scheme":"Other"},{"subitem_subject":"Populus maximowiczii×Populus plantierensis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Growth of Suspension Cultured Cell of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii×Populus plantierensis in NaCl Containing Medium","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Growth of Suspension Cultured Cell of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii×Populus plantierensis in NaCl Containing Medium"}]},"item_type_id":"4","owner":"1","path":["2538","2537"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25377","relation_version_is_last":true,"title":["Growth of Suspension Cultured Cell of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii×Populus plantierensis in NaCl Containing Medium"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:00:59.278648+00:00"}