{"created":"2021-03-01T06:43:20.204249+00:00","id":25382,"links":{},"metadata":{"_buckets":{"deposit":"30c6cd18-63df-47fd-9562-317a4480b190"},"_deposit":{"id":"25382","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25382"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025382","sets":["27:2466:2467:2539","9:504:2469:2540"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Mechanism of Secondary Metabolite Production in Poplar Suspension Cell Cultures"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"134","bibliographicPageStart":"123","bibliographicVolumeNumber":"101","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ポプラ懸濁培養細胞にエリシターとしてペクチナーゼを添加するとガスクロマトグラム上に二次代謝物のピークが見られた。そこでこれらの二次代謝物生成機構を明らかにするために，活性酸素関連試薬，不飽和脂肪酸類を添加してストレス特異的二次代謝物生成への影響を検討した。活性酸素生成試薬としてジギトニン，ドキソルビシン，活性酸素種として過酸化水素，活性酸素消去剤としてアスコルビン酸，グルタチオン，カタラーゼを用いた。ジギトニン，過酸化水素を添加するとそれぞれ，ペクチナーゼ処理の約63％，15％にあたる二次代謝物の生成が見られた。また不飽和脂肪酸としてリノール酸，リノレン酸，アラキドン酸，エイコサペンタエン酸を用いた。リノレン酸の添加により，ペクチナーゼ処理の約13％の二次代謝物生成が見られた。以上のことからポプラ懸濁培養細胞におけるストレス特異的二次代謝物生成機構に活性酸素と不飽和脂肪酸が関与していることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Pectinase elicits the production of some secondary metabolites in poplar suspension cell cultures. We have already reported that one of these metabolites is trichadonic acid. To understand the mechanism of this pectinase-induced production of these metabolites, the effect of active oxygen species (AOS) and unsaturated fatty acids was investigated for the induction of the main products (unknown compounds X). The reagents used were digitonin and doxorubicin as AOS-generating reagents, hydrogen peroxide as AOS, ascorbic acid and glutatione as AOS-scavengers, and catalase. As unsaturated fatty acids, linoleic acid, linolenic acid, arachidonic acid and eicosapentaenoic acid were added. The amount of (X) induced by the addition of digitonin and hydrogen peroxide reached 63% and 15% respectively compared to that of pectinase treatment. And by addition of linolenic acid, it reached 13%. These results suggest that AOS and unsaturated fatty acid might be concerned with pectinase-induced production of secondary metabolites.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54400","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SATO, Mari"}]},{"nameIdentifiers":[{"nameIdentifier":"54401","nameIdentifierScheme":"WEKO"}],"names":[{"name":"INOUE, Hiroki"}]},{"nameIdentifiers":[{"nameIdentifier":"54402","nameIdentifierScheme":"WEKO"}],"names":[{"name":"KAMODA, Shigehiro"}]},{"nameIdentifiers":[{"nameIdentifier":"54403","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TERADA, Tamami"}]},{"nameIdentifiers":[{"nameIdentifier":"54404","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SABURI, Yoshimasa"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"東京大学農学部附属演習林北海道演習林"},{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"University Forest in Hokkaido, Faculty of Agriculture, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 万里"}],"nameIdentifiers":[{"nameIdentifier":"54395","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"井上, 広喜"}],"nameIdentifiers":[{"nameIdentifier":"54396","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鴨田, 重裕"}],"nameIdentifiers":[{"nameIdentifier":"54397","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"寺田, 珠実"}],"nameIdentifiers":[{"nameIdentifier":"54398","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐分, 義正"}],"nameIdentifiers":[{"nameIdentifier":"54399","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh101004.pdf","filesize":[{"value":"721.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh101004.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25382/files/esrh101004.pdf"},"version_id":"4a400913-3a1f-4d85-9a26-5a4d064d87d0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ポプラ培養細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"二次代謝物","subitem_subject_scheme":"Other"},{"subitem_subject":"ペクチナーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"活性酸素","subitem_subject_scheme":"Other"},{"subitem_subject":"不飽和脂肪酸","subitem_subject_scheme":"Other"},{"subitem_subject":"Poplar cell cultures","subitem_subject_scheme":"Other"},{"subitem_subject":"Secondary metabolites","subitem_subject_scheme":"Other"},{"subitem_subject":"Pectinase","subitem_subject_scheme":"Other"},{"subitem_subject":"Active oxygen","subitem_subject_scheme":"Other"},{"subitem_subject":"Unsaturated fatty acid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ポプラ懸濁培養細胞における二次代謝物生成機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ポプラ懸濁培養細胞における二次代謝物生成機構"}]},"item_type_id":"4","owner":"1","path":["2540","2539"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25382","relation_version_is_last":true,"title":["ポプラ懸濁培養細胞における二次代謝物生成機構"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:00:55.981756+00:00"}