{"created":"2021-03-01T06:43:21.500331+00:00","id":25402,"links":{},"metadata":{"_buckets":{"deposit":"64582354-3978-4f90-9c5a-6ab4ae23086b"},"_deposit":{"id":"25402","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25402"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025402","sets":["27:2466:2467:2543","9:504:2469:2544"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Antimicrobial Activities of Extractives from the Barks of Japanese Coniferous Trees"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"233","bibliographicPageStart":"219","bibliographicVolumeNumber":"99","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では，6種の日本産有用針葉樹（トドマツ：Abies sachalinensis Fr. Schm.，アカエゾマツ：Picea glehnii Fr. Schm.，アカマツ：Pinus densiflora Sieb. et Zucc.，カラマツ：Larix leptolepis Sieb. et Zucc.，ヒノキ：Chamaecyparis obtusa Sieb. et Zucc.，スギ：Cryptomeria japonica D. Don）の内樹皮より得た抽出物の細菌，放線菌および糸状菌に対する抗菌活性を調べ，さらに原因となる物質を検索した。内樹皮粉を，n－ヘキサン，酢酸エチル，エタノールを用いて逐次抽出し，得られた抽出物について抗菌試験を行ったところ，細菌（Pseudomonas paucimobilis），放線菌（Nocardia autotropica，N. globerura，N. corallina）に対しては，いずれの樹種のn－ヘキサン抽出物とも抗菌活性を示した。さらにこの中で，アカエゾマツ，およびヒノキのn－ヘキサン抽出物では特に強い抗菌性が認められた。これに対して，いずれの樹種の酢酸エチル抽出物，およびエタノール抽出物とも細菌，放線菌に対する抗菌活性はn－ヘキサン抽出物に比べて著しく低かった。さらに，アカエゾマツのn－ヘキサン抽出物について抗菌性の原因物質を調べたところ，これらの菌に対する抗菌物質の一つとしてジテルペンカルボン酸のdehydroabietic acidであるとが明らかとなった。一方，糸状菌（Phanerochaete chrysosporium, Trichoderma reesei）に対して内樹皮抽出物の抗菌性を調べた結果では，細菌，放線菌の場合とは対照的にn－ヘキサン抽出物には抗菌性が認められず，逆に細菌，放線菌に対して抗菌性の弱かった酢酸エチル抽出物，エタノール抽出物に抗菌性が認められた。特に，アカエゾマツの酢酸エチル抽出物の抗菌性は非常に強いことから，特異的な抗菌物質の存在が示唆された。そこで，この抗菌物質について検索したところ，スチルベン配糖体の一種であるisorhapontinが主な原因物質であることが明らかとなった。","subitem_description_type":"Abstract"},{"subitem_description":"Antimicrobial activities of extracts from the inner bark of six industrially important coniferous species in Japan were examined. Air dried inner bark was ground to pass a 1 mm sieve, and extracted successively with n-hexane, ethyl acetate, and ethanol at room temperature. Extract with n-hexane from all species used in this study inhibited significantly growth of both Pseudomonas paucimobilis and three species of Nocardia, which were little inhibited by the extracts with ethyl acetate and ethanol. Growth of microorganisms was completely inhibited by the addition of 0.1% (w/w) n-hexane extracts from Picea glehnii and Chamaecyparis obtusa on an agar medium. A major component responsible for the inhibition of growth of P. paucimobilis and Nocardia spp. was purified and identified as dehydroabietic acid (I). In contrast to the results for P. paucimobilis and Nocardia spp., n-hexane extracts showed no inhibition on the growth of two fungal species (Phanerochaete chrysosporium and Trichoderma reesei). The growth of these fungi on an agar medium was however inhibited around a paper disk containing ethanol extracts. Ethyl acetate extracts from the inner bark of P. glehnii inhibited the growth of the two fungi. The extracts was purified and isorhapontin (II), a stilbene glucoside, was identified as the major component in the most active fraction.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54508","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SHIBUTANI, Sakae"}]},{"nameIdentifiers":[{"nameIdentifier":"54509","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SAMEJIMA, Masahiro"}]},{"nameIdentifiers":[{"nameIdentifier":"54510","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SABURI, Yoshimasa"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"澁谷, 栄"}],"nameIdentifiers":[{"nameIdentifier":"54505","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鮫島, 正浩"}],"nameIdentifiers":[{"nameIdentifier":"54506","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐分, 義正"}],"nameIdentifiers":[{"nameIdentifier":"54507","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh099011.pdf","filesize":[{"value":"941.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh099011.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25402/files/esrh099011.pdf"},"version_id":"95f4245c-a4cf-4ebd-bf13-2a4e6d18802f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"樹皮","subitem_subject_scheme":"Other"},{"subitem_subject":"抽出成分","subitem_subject_scheme":"Other"},{"subitem_subject":"抗菌活性","subitem_subject_scheme":"Other"},{"subitem_subject":"樹脂酸","subitem_subject_scheme":"Other"},{"subitem_subject":"スチルベン","subitem_subject_scheme":"Other"},{"subitem_subject":"Bark","subitem_subject_scheme":"Other"},{"subitem_subject":"Extractives","subitem_subject_scheme":"Other"},{"subitem_subject":"Antimicrobial activity","subitem_subject_scheme":"Other"},{"subitem_subject":"Resin acid","subitem_subject_scheme":"Other"},{"subitem_subject":"Stilbene","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"日本産針葉樹樹皮抽出成分の抗菌活性について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"日本産針葉樹樹皮抽出成分の抗菌活性について"}]},"item_type_id":"4","owner":"1","path":["2544","2543"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25402","relation_version_is_last":true,"title":["日本産針葉樹樹皮抽出成分の抗菌活性について"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:40.671592+00:00"}