{"created":"2021-03-01T06:43:22.928194+00:00","id":25424,"links":{},"metadata":{"_buckets":{"deposit":"451514af-d7fd-4121-849b-69b1f908b424"},"_deposit":{"id":"25424","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25424"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025424","sets":["27:2466:2467:2549","9:504:2469:2550"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"イチョウ培養系における葉柄及び胚の植物ホルモン応答"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"123","bibliographicPageStart":"119","bibliographicVolumeNumber":"96","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Petioles and embryos of Ginkgo biloba were cultured on modified Linsmaier & Skoog medium supplemented with various kinds and concentrations of auxins and cytokinins. Calli were easily obtained on the medium supplemented with various combinations of 2,4-dichlorophenoxyacetic acid or naphthaleneacetic acid as an auxin, and kinetin or 6-benzyladenine as a cytokinin. The calli grew rapidly and were able to be subcultured on the same medium. These calli were white in color in the dark, but exposed to light, they easily changed to green. Organogenesis was not observed in petiole explants, but embryos excised from the seeds immediately after their falling to the ground showed germination and leaf expansion on the medium supplemented with indoleacetic acid (IAA) or indolebutyric acid (IBA) alone. When embryos excised from the seeds stored at 3℃ for about 3 months were used, they germinated and seedlings were obtained not only on IAA or IBA containing medium but also on a hormone-free one. Redifferentiation from calli was not seen by combining of growth regulators or modifying the mineral salt in the medium.","subitem_description_type":"Abstract"},{"subitem_description":"イチョウの葉柄ならびに胚を外植片として用い，改変Linsmaier & Skoog培地にオーキシンとサイトカイニンの種類と濃度を変えて添加した培地で培養した。オーキシンとして2,4-DまたはNAA，サイトカイニンとしてBAまたはカイネチンを組み合わせて添加すると脱分化が容易に起こり成長の速いカルスが得られ，継代して培養が可能であった。このカルスは，暗所では白色をしているが光照射により容易に緑化した。葉柄からの分化は観察されていないが，落下直後の果実から取りだした胚をIAAまたはIBAのみを加えた培地で培養したところ，発芽して葉の展開が見られる場合があった。また，3℃で3カ月間保存した種子から取りだした胚を用いた場合，ホルモンを加えない培地においても発芽がみられ完全な幼植物体になるものもあった。一方，カルスからの再分化は，成長調節物質の組み合わせや培地の無機塩の改変によっては認められなかった。","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54654","nameIdentifierScheme":"WEKO"}],"names":[{"name":"井上, 広喜"}]},{"nameIdentifiers":[{"nameIdentifier":"54655","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐藤, 征嗣"}]},{"nameIdentifiers":[{"nameIdentifier":"54656","nameIdentifierScheme":"WEKO"}],"names":[{"name":"鴨田, 重裕"}]},{"nameIdentifiers":[{"nameIdentifier":"54657","nameIdentifierScheme":"WEKO"}],"names":[{"name":"寺田, 珠実"}]},{"nameIdentifiers":[{"nameIdentifier":"54658","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐分, 義正"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院農学生命科学研究科生物材料科学専攻"},{"subitem_text_value":"東京大学農学部附属演習林研究部"},{"subitem_text_value":"Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo"},{"subitem_text_value":"Research Division of the University Forests, Faculty of Agriculture, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"INOUE, Hiroki"}],"nameIdentifiers":[{"nameIdentifier":"54649","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SATO, Seiji"}],"nameIdentifiers":[{"nameIdentifier":"54650","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KAMODA, Shigehiro"}],"nameIdentifiers":[{"nameIdentifier":"54651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TERADA, Tamami"}],"nameIdentifiers":[{"nameIdentifier":"54652","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SABURI, Yoshimasa"}],"nameIdentifiers":[{"nameIdentifier":"54653","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh096005.pdf","filesize":[{"value":"348.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh096005.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25424/files/esrh096005.pdf"},"version_id":"4fbedff1-dca6-464f-8564-1433a37da71d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Ginkgo biloba","subitem_subject_scheme":"Other"},{"subitem_subject":"phytohormone","subitem_subject_scheme":"Other"},{"subitem_subject":"dedifferentiation","subitem_subject_scheme":"Other"},{"subitem_subject":"germination","subitem_subject_scheme":"Other"},{"subitem_subject":"イチョウ","subitem_subject_scheme":"Other"},{"subitem_subject":"植物ホルモン","subitem_subject_scheme":"Other"},{"subitem_subject":"脱分化","subitem_subject_scheme":"Other"},{"subitem_subject":"発芽","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hormonal Responses of Petioles and Embryos in Ginkgo biloba Cultures","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hormonal Responses of Petioles and Embryos in Ginkgo biloba Cultures"}]},"item_type_id":"4","owner":"1","path":["2550","2549"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25424","relation_version_is_last":true,"title":["Hormonal Responses of Petioles and Embryos in Ginkgo biloba Cultures"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:40.308096+00:00"}