{"created":"2021-03-01T06:43:23.662318+00:00","id":25435,"links":{},"metadata":{"_buckets":{"deposit":"c0ee167d-8d32-464f-934d-a4bffe9c4aba"},"_deposit":{"id":"25435","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25435"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025435","sets":["27:2466:2467:2553","9:504:2469:2554"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Plant Regeneration and Mass Propagation of Betula tatewakiana by Winter Bud Culture"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"15","bibliographicPageStart":"9","bibliographicVolumeNumber":"94","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヤチカンバの冬芽をBAP0.8mg/lを添加したWPM上で培養することによりシュートを伸長させ，さらにそのシュートを切り取って，IBA0.5mg/lとNAAを0.02mg/l添加した1/2MS培地にさしつけることで，発根させ幼植物体を再生させることができた。また，再生した幼植物体の茎軸，葉柄および腋芽を，BAP0.8mg/lとGA3 5.0mg/lを組み合わせて添加したWPMで培養したところ，BAPを添加した培地で，腋芽からマルチプルシュートを誘導することができ，種苗の大量増殖が可能であることが明らかになった。また，GA3の添加は，BAPと併用した場合にシュート数の増加に有効であった。","subitem_description_type":"Abstract"},{"subitem_description":"Winter buds of Betula tatewakiana were cultured on WPM containing 0.8mg/l of BAP. Shoots were successfully elongated from the buds on the medium. The shoots were cut and inoculated on to 1/2MS containing 0.5mg/l of IBA and 0.02mg/l of NAA. They were successfully rooted on the medium and regenerated perfect plantlets. Internodal segments, petioles and axillary buds were isolated from the plantlet and cultured on a medium containing 0.8mg/l of BAP and 5.0mg/l of GA3. Multiple shoots were elongated from the axillary buds cultured on a medium containing 0.8mg/l of BAP. GA3 concentration in the medium was effective in the proliferation of shoots when it was added with BAP.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54708","nameIdentifierScheme":"WEKO"}],"names":[{"name":"IDE, Yuji"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学農学部附属演習林研究部"},{"subitem_text_value":"Research Division of the University Forests, Faculty of Agriculture, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"井出, 雄二"}],"nameIdentifiers":[{"nameIdentifier":"54707","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh094002.pdf","filesize":[{"value":"761.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh094002.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25435/files/esrh094002.pdf"},"version_id":"097fae2e-be3e-42a1-a72c-2277a86fde15"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ヤチカンバ","subitem_subject_scheme":"Other"},{"subitem_subject":"組織培養","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子保存","subitem_subject_scheme":"Other"},{"subitem_subject":"大量増殖","subitem_subject_scheme":"Other"},{"subitem_subject":"Betula tatewakiana","subitem_subject_scheme":"Other"},{"subitem_subject":"Tissue culture","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene conservation","subitem_subject_scheme":"Other"},{"subitem_subject":"Mass propagation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヤチカンバ（Betula tatewakiana）冬芽の培養による植物体再生と大量増殖法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヤチカンバ（Betula tatewakiana）冬芽の培養による植物体再生と大量増殖法の確立"}]},"item_type_id":"4","owner":"1","path":["2554","2553"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25435","relation_version_is_last":true,"title":["ヤチカンバ（Betula tatewakiana）冬芽の培養による植物体再生と大量増殖法の確立"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:41.545647+00:00"}