{"created":"2021-03-01T06:43:24.566939+00:00","id":25449,"links":{},"metadata":{"_buckets":{"deposit":"3aecf4b5-2c5f-4720-aa89-2eb7202b7124"},"_deposit":{"id":"25449","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25449"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025449","sets":["27:2466:2467:2557","9:504:2469:2558"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"L-Phenylalanine Ammonia-Lyase Activities in Cell Suspension Cultures of Eucalyptus polybractea (II) : Effects of media components on L-phenylalanine ammonia-lyase activities"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographicVolumeNumber":"92","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ユーカリ属懸濁培養細胞を用いて，培地構成成分が，細胞増殖とPAL活性に与える影響を調べた。糖濃度を増加させて細胞を培養したところ，増殖が阻害され，PAL活性は高く誘導された。また窒素源濃度の増加も細胞増殖の抑制とPAL活性の誘導を引き起こしたが，細胞中の総フェノール量はかえって減少していた。リン源を増やすと細胞増殖は促進され，PAL活性が抑制された。さらに細胞分裂阻害剤であるアフィディコリンの添加により細胞増殖を抑制すると，PAL活性が誘導され，その活性のピークはしばらくの間保持された。以上のことから，E. polybractea培養細胞におけるPAL活性の誘導には，フェノール生成よりも細胞増殖が関係しているらしいことが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Effects of media components on cell growth and PAL activity in cell suspension cultures of Eucalyptus polybractea were investigated. Both high sugar and high nitrate concentrations caused a suppresion of cell growth and an increase in PAL activity. PAL activity was induced significantly, however, total phenolics contents were depressed in the case of high nitrate. High phosphate concentration decreased PAL activity, while promoted cell growth. Furthermore, addition of aphidicolin (cell division regulator) to the medium reduced cell growth and increased PAL activity. And the high level of PAL activity was kept for a few days. These observasions indicate that the level of PAL activity in the cell cultures of E. polybractea varies in response to cell growth not to phenolics accumulation.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54781","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TERADA, Tamami"}]},{"nameIdentifiers":[{"nameIdentifier":"54782","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YAMAGUCHI, Takashi"}]},{"nameIdentifiers":[{"nameIdentifier":"54783","nameIdentifierScheme":"WEKO"}],"names":[{"name":"YOSHIMOTO, Tomotaka"}]},{"nameIdentifiers":[{"nameIdentifier":"54784","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SABURI, Yoshimasa"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学農学部林産学科"},{"subitem_text_value":"現所属（財）杉山産業化学研究所"},{"subitem_text_value":"現所属（財）自然農法国際研究開発センター"},{"subitem_text_value":"Department of Forest Products, Faculty of Agriculture, The University of Tokyo"},{"subitem_text_value":"Sugiyama Chemical and Industrial Laboratory"},{"subitem_text_value":"Nature Farming International Research Foundation"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"寺田, 珠実"}],"nameIdentifiers":[{"nameIdentifier":"54777","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山口, 隆司"}],"nameIdentifiers":[{"nameIdentifier":"54778","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"善本, 知孝"}],"nameIdentifiers":[{"nameIdentifier":"54779","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐分, 義正"}],"nameIdentifiers":[{"nameIdentifier":"54780","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh092001.pdf","filesize":[{"value":"468.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh092001.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25449/files/esrh092001.pdf"},"version_id":"0c482673-c6ac-403b-9aa2-c67313843e01"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ユーカリ培養細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"PAL","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞増殖","subitem_subject_scheme":"Other"},{"subitem_subject":"培地構成成分","subitem_subject_scheme":"Other"},{"subitem_subject":"Eucalyptus polybractea cell cultures","subitem_subject_scheme":"Other"},{"subitem_subject":"PAL","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell growth","subitem_subject_scheme":"Other"},{"subitem_subject":"Media components","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ユーカリ属培養細胞におけるフェニルアラニンアンモニアリア-ゼ活性（第2報） : 培地構成成分の影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ユーカリ属培養細胞におけるフェニルアラニンアンモニアリア-ゼ活性（第2報） : 培地構成成分の影響"}]},"item_type_id":"4","owner":"1","path":["2558","2557"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25449","relation_version_is_last":true,"title":["ユーカリ属培養細胞におけるフェニルアラニンアンモニアリア-ゼ活性（第2報） : 培地構成成分の影響"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:41.214549+00:00"}