{"created":"2021-03-01T06:43:25.622609+00:00","id":25465,"links":{},"metadata":{"_buckets":{"deposit":"a6a65e0a-2c59-4a15-b5c1-214e0bda5793"},"_deposit":{"id":"25465","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25465"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025465","sets":["27:2466:2467:2561","9:504:2469:2562"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"アカシアマンギウムの種々の試験管内培養組織からのプロトプラストの単離（英文）"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"21","bibliographicPageStart":"17","bibliographicVolumeNumber":"90","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Various enzyme combinations were tested for protoplast isolation from compound leaves of in vitro cultured shoots of Acacia mangium. The combination of 1% Cellulase 'ONOZUKA'RS, 0.2% Pectolyase Y-23 and 2% Driselase was optimal to obtain a high yields of protoplasts. The protoplasts were also successfully isolated from compound leaves and cotyledons of young seedlings and callus, but not from phyllode.","subitem_description_type":"Abstract"},{"subitem_description":"アカシアマンギウムの培養シュートの本葉からプロトプラストを単離する酵素組成を検索した。酵素液は，セルラーゼ“オノズカ”RS1％，ペクトリアーゼY-23，0.2％，ドリセラーゼ2％の添加が適当と判断された。また，芽生えの本葉，子葉およびカルスからプロトプラストを単離できたが，仮葉からは単離できなかった。","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54865","nameIdentifierScheme":"WEKO"}],"names":[{"name":"齋藤, 陽子"}]},{"nameIdentifiers":[{"nameIdentifier":"54866","nameIdentifierScheme":"WEKO"}],"names":[{"name":"井出, 雄二"}]},{"nameIdentifiers":[{"nameIdentifier":"54867","nameIdentifierScheme":"WEKO"}],"names":[{"name":"小島, 克己"}]},{"nameIdentifiers":[{"nameIdentifier":"54868","nameIdentifierScheme":"WEKO"}],"names":[{"name":"佐々木, 惠彦"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Forestry, Faculty of Agriculture, The University of Tokyo"},{"subitem_text_value":"Research Division of the University Forests, Faculty of Agriculture, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SAITO, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"54861","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"IDE, Yuji"}],"nameIdentifiers":[{"nameIdentifier":"54862","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KOJIMA, Katsumi"}],"nameIdentifiers":[{"nameIdentifier":"54863","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"SASAKI, Satohiko"}],"nameIdentifiers":[{"nameIdentifier":"54864","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh090002.pdf","filesize":[{"value":"441.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh090002.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25465/files/esrh090002.pdf"},"version_id":"2ef2146b-b150-4f29-9610-dc9732f8e46f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Acacia mangium","subitem_subject_scheme":"Other"},{"subitem_subject":"Protoplast","subitem_subject_scheme":"Other"},{"subitem_subject":"Isolation","subitem_subject_scheme":"Other"},{"subitem_subject":"アカシアマンギウム","subitem_subject_scheme":"Other"},{"subitem_subject":"プロトプラスト","subitem_subject_scheme":"Other"},{"subitem_subject":"単離","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation of Protoplasts from Various Tissues of Acacia mangium Cultured in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation of Protoplasts from Various Tissues of Acacia mangium Cultured in vitro"}]},"item_type_id":"4","owner":"1","path":["2562","2561"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25465","relation_version_is_last":true,"title":["Isolation of Protoplasts from Various Tissues of Acacia mangium Cultured in vitro"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:41.919532+00:00"}