{"created":"2021-03-01T06:43:26.878369+00:00","id":25484,"links":{},"metadata":{"_buckets":{"deposit":"21b15835-44ab-42f7-b468-24aa64f24485"},"_deposit":{"id":"25484","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25484"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025484","sets":["27:2466:2467:2565","9:504:2469:2566"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Polyamines in Relation to Growth in Cell Suspension Cultures of Eucalyptus polybractea (II) : Polyamines and cell division"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"163","bibliographicPageStart":"157","bibliographicVolumeNumber":"88","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"MGBGを添加して生重量，細胞数，DNA量いずれの増加も抑制したユーカリ培養細胞にSpdあるいはSpmを添加すると，まずDNA量が急激に増加し，1～2日遅れて細胞数が増加した。またSpmよりもSpdの方が増殖回復に有効であった。MGBGの添加により細胞周期のS期付近で停止したユーカリの細胞分裂が，Spdの添加で再び進行したものと考えられ，この方法で同調培養系の確立も可能性があるものと思われる。細胞分裂（S期）制御剤のアフィディコリンを用いた場合，アフィディコリンを解除すると細胞数の増加に先立ちポリアミン量の増加が観察されたことからも，細胞分裂へのポリアミンの関与が示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Addition of Spd or Spm to a medium containing MGBG, which reduced the cell growth (fw, cell number, DNA content) of Eucalyptus polybractea cultured cells, could restore the DNA content first, and cell number 1-2 days later. Spd was more effective for restoration from growth inhibition than Spm. Cell division was arrested in the neighborhood of the S phase of the cell cycle by MGBG and Spd operated the cell cycle again. Therefore, synchronous cultures may be established using MGBG in combination with Spd. In the case of the treatment of cell division (S phase) regulator (aphidicolin), after removal of the block, levels of polyamines increased prior to the increase of cell number. These results suggest that polyamines correlate to cell division.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"54984","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TERADA, Tamami"}]},{"nameIdentifiers":[{"nameIdentifier":"54985","nameIdentifierScheme":"WEKO"}],"names":[{"name":"TAKAYAMA, Satoru"}]},{"nameIdentifiers":[{"nameIdentifier":"54986","nameIdentifierScheme":"WEKO"}],"names":[{"name":"SABURI, Yoshimasa"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学農学部林産学科"},{"subitem_text_value":"Department of Forest Products, Faculty of Agriculture, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"寺田, 珠実"}],"nameIdentifiers":[{"nameIdentifier":"54981","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高山, 悟"}],"nameIdentifiers":[{"nameIdentifier":"54982","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐分, 義正"}],"nameIdentifiers":[{"nameIdentifier":"54983","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh088010.pdf","filesize":[{"value":"386.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh088010.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25484/files/esrh088010.pdf"},"version_id":"ecad11cf-147a-4a58-b761-3e8164abd701"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ユーカリ","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞分裂","subitem_subject_scheme":"Other"},{"subitem_subject":"S期","subitem_subject_scheme":"Other"},{"subitem_subject":"MGBG","subitem_subject_scheme":"Other"},{"subitem_subject":"Spd","subitem_subject_scheme":"Other"},{"subitem_subject":"Eucalyptus polybractea","subitem_subject_scheme":"Other"},{"subitem_subject":"cell division","subitem_subject_scheme":"Other"},{"subitem_subject":"S phse","subitem_subject_scheme":"Other"},{"subitem_subject":"MGBG","subitem_subject_scheme":"Other"},{"subitem_subject":"Spd","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ユーカリ細胞培養系におけるポリアミンと増殖の関係（2） : ポリアミンと細胞分裂","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ユーカリ細胞培養系におけるポリアミンと増殖の関係（2） : ポリアミンと細胞分裂"}]},"item_type_id":"4","owner":"1","path":["2566","2565"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25484","relation_version_is_last":true,"title":["ユーカリ細胞培養系におけるポリアミンと増殖の関係（2） : ポリアミンと細胞分裂"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:52:47.013692+00:00"}