{"created":"2021-03-01T06:43:27.985361+00:00","id":25501,"links":{},"metadata":{"_buckets":{"deposit":"4bc8e6b8-d68f-4768-9e67-0c244de556af"},"_deposit":{"id":"25501","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25501"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00025501","sets":["27:2466:2467:2571","9:504:2469:2572"]},"item_4_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"クヌギの培養シュートからのプロトプラストの単離と培養"}]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"47","bibliographicPageStart":"43","bibliographicVolumeNumber":"85","bibliographic_titles":[{"bibliographic_title":"東京大学農学部演習林報告"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"クヌギの若い実生の節間部の小片の培養により，えき芽から伸長させたシュートから，酵素液を用いてプロトプラストを単離した。0.5gのシュート組織から約1×105個のプロトプラストが得られた。このプロトプラストを，2,4-D（2,4-dichlorophenoxyacetic acid）3mg/lを含んだ改変MS（Murashige and Skoog, 1962）培地に約1×105/mlの密度で懸濁して培養した。24時間後に最初のバディングが観察された。バディングはさらに3週間にわたり連続的に起こった。","subitem_description_type":"Abstract"},{"subitem_description":"Protoplasts were isolated from shoot tissues which were derived from culture of nodal segments of Quercus acutissima young seedlings by an enzyme solution. About 1×105 protoplasts were isolated from 0.5g of shoot tissue. The protoplasts were cultured in the liquid modified MS medium containing 3mg/l of 2,4-D (2,4-dichlorophenoxyacetic acid) at the density of 1×105/ml. First cell buddings were observed after 24hr of protoplast culture, and cell buddings were continued three weeks.","subitem_description_type":"Abstract"}]},"item_4_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"55072","nameIdentifierScheme":"WEKO"}],"names":[{"name":"井出, 雄二"}]},{"nameIdentifiers":[{"nameIdentifier":"55073","nameIdentifierScheme":"WEKO"}],"names":[{"name":"山本, 茂弘"}]},{"nameIdentifiers":[{"nameIdentifier":"55074","nameIdentifierScheme":"WEKO"}],"names":[{"name":"近藤, 晃"}]}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東京大学大学院農学生命科学研究科附属演習林"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00162451","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03716007","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"610","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"The Tokyo University Forests"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学農学部附属演習林研究部"},{"subitem_text_value":"静岡県林業技術センター"},{"subitem_text_value":"Research Division of The University Forests, Faculty of Agriculture, The University of Tokyo"},{"subitem_text_value":"Shizuoka Prefecture Forestry Technology Center"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"IDE, Yuji"}],"nameIdentifiers":[{"nameIdentifier":"55069","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"YAMAMOTO, Shigehiro"}],"nameIdentifiers":[{"nameIdentifier":"55070","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KONDO, Akira"}],"nameIdentifiers":[{"nameIdentifier":"55071","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-09"}],"displaytype":"detail","filename":"esrh085004.pdf","filesize":[{"value":"506.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"esrh085004.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/25501/files/esrh085004.pdf"},"version_id":"f10b7ddc-6313-4d2f-a37a-dcbc4016b0cb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"クヌギ","subitem_subject_scheme":"Other"},{"subitem_subject":"培養シュート","subitem_subject_scheme":"Other"},{"subitem_subject":"プロトプラスト","subitem_subject_scheme":"Other"},{"subitem_subject":"バディング","subitem_subject_scheme":"Other"},{"subitem_subject":"Quercus acutissima","subitem_subject_scheme":"Other"},{"subitem_subject":"Shoot culture","subitem_subject_scheme":"Other"},{"subitem_subject":"Protoplast","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell budding","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation and Culture of Protoplasts from Quercus acutissima Shoot Cultures","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation and Culture of Protoplasts from Quercus acutissima Shoot Cultures"}]},"item_type_id":"4","owner":"1","path":["2572","2571"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-09-03"},"publish_date":"2014-09-03","publish_status":"0","recid":"25501","relation_version_is_last":true,"title":["Isolation and Culture of Protoplasts from Quercus acutissima Shoot Cultures"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:03:42.434732+00:00"}