{"created":"2021-03-01T06:20:05.242271+00:00","id":3446,"links":{},"metadata":{"_buckets":{"deposit":"cd2f48a0-cf71-4f67-a681-45385a268eef"},"_deposit":{"id":"3446","owners":[],"pid":{"revision_id":0,"type":"depid","value":"3446"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00003446","sets":["110:246:247","9:233:234"]},"item_7_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-09-27","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_7_date_granted_25":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2011-09-27"}]},"item_7_degree_name_20":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"修士(科学)"}]},"item_7_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A large fraction of mitochondrial proteins are cleaved upon entry into the mitochondria, but prediction of this cleavage is still challenging. In chapter 1, I summarize necessary background to this important problem. In chapter 2, I demonstrate that my system, Mitochondrial matrix targeting Signal Predictor, MoiraiSP, which is based on data from recent proteomic studies can correctly identified the cleavage site of MPP more than 75% accuracy in both plant and yeast dataset. In chapter 3, I introduce sequence divergence, LD(i), as a novel feature for sorting signal prediction, and show that prediction can be improved by LD(i) than random, especially with other famous features such as physico-chemical propensities. In chapter 4, I present that MoiraiSP can treat a related problem, predicting mitochondrial matrix targeting signal by using only N-terminal sequence information such as net-charge or LD(i). MoiraiSP discriminates between cleaved and non-cleaved mitochondrial proteins with a success rate of 97% (plant) or 91% (yeast) by cross validation. Finally, in chapter 5, I discuss some novel candidates of protease substrates, which came up during my work.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"8267","nameIdentifierScheme":"WEKO"}],"names":[{"name":"深沢, 嘉紀"}]}]},"item_7_select_21":{"attribute_name":"学位","attribute_value_mlt":[{"subitem_select_item":"master"}]},"item_7_subject_13":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"463","subitem_subject_scheme":"NDC"}]},"item_7_text_24":{"attribute_name":"研究科・専攻","attribute_value_mlt":[{"subitem_text_value":"新領域創成科学研究科情報生命科学専攻"}]},"item_7_text_27":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_value":"修創域第4109号"}]},"item_7_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院新領域創成科学研究科情報生命科学専攻"},{"subitem_text_value":"Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fukasawa, Yoshinori"}],"nameIdentifiers":[{"nameIdentifier":"8266","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-31"}],"displaytype":"detail","filename":"K-03095.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文(fulltext)","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/3446/files/K-03095.pdf"},"version_id":"3e62c31e-6da2-494e-90a9-74b5a63b3727"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-31"}],"displaytype":"detail","filename":"K-03095-a.pdf","filesize":[{"value":"70.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨(summary)","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/3446/files/K-03095-a.pdf"},"version_id":"37bbac01-48cc-4109-ae3a-56b08ea384c7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mitochondria","subitem_subject_scheme":"Other"},{"subitem_subject":"ミトコンドリア","subitem_subject_scheme":"Other"},{"subitem_subject":"Targeting signal","subitem_subject_scheme":"Other"},{"subitem_subject":"clearage","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Development of a novel mitochondrial cleavage site predictor","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development of a novel mitochondrial cleavage site predictor"}]},"item_type_id":"7","owner":"1","path":["234","247"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-10-10"},"publish_date":"2012-10-10","publish_status":"0","recid":"3446","relation_version_is_last":true,"title":["Development of a novel mitochondrial cleavage site predictor"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:45:08.175179+00:00"}