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Exploring Cellular Targets of Vanillin Based on Morphological Changes of Saccharomyces cerevisiae
http://hdl.handle.net/2261/48924
http://hdl.handle.net/2261/48924ca35b3e8-cf0a-44c9-b34d-3de10a6fe41e
名前 / ファイル | ライセンス | アクション |
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本文(fulltext) (1.7 MB)
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要旨(summary) (317.9 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2012-10-12 | |||||
タイトル | ||||||
タイトル | Exploring Cellular Targets of Vanillin Based on Morphological Changes of Saccharomyces cerevisiae | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Saccharomyces cerevisiae | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | vanillin | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | target-prediction | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | cell morphology | |||||
主題Scheme | Other | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_46ec | |||||
タイプ | thesis | |||||
その他のタイトル | ||||||
その他のタイトル | 出芽酵母の形態変化に基づくvanillinの細胞内標的探索 | |||||
著者 |
Suga, Yohei
× Suga, Yohei |
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著者別名 | ||||||
識別子 | 8776 | |||||
識別子Scheme | WEKO | |||||
姓名 | 菅, 洋平 | |||||
著者所属 | ||||||
著者所属 | 東京大学大学院新領域創成科学研究科生命科学研究系先端生命科学専攻 | |||||
著者所属 | ||||||
著者所属 | Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo | |||||
Abstract | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Vanillin is one of the major phenolic compounds degraded from lignin. It is considered as a problematic byproduct of bioethanol production from lignocelluloses since it inhibits yeast growth and fermentation. However, detailed inhibitory mechanisms of vanillin are still unknown. In this study, I investigated intercellular targets of vanillin based on the image profiling method to infer the drug targets developed recently (Ohnuki et al., 2010). Using this method, I revealed that the morphology of wild-type cells treated with vanillin is similar with that of erg6Δ and his3Δ adh6Δ treated with vanillin. From the result of erg6Δ, the mutant of elgosterol-biosynthesis, it was suggested that vanillin does not affect its pathway. From the result of his3Δ adh6Δ, the mutant of vanillin-conversion, it was suggested that the accuracy of the image profiling was increased probably due to the inability of vanillin bio-conversion of his3Δ adh6Δ, which makes the effects of vanillin more aggravate. In common with the three strains, cell sizes were reduced in the presence of vanillin in a dose-dependent manner and significant enrichment of gene ontology term "ribosomal large subunit" was detected in the inference results. In conclusion, these results suggest that vanillin affects ribosome or related components in yeast cells and induces growth defect. | |||||
書誌情報 | 発行日 2011-03-24 | |||||
日本十進分類法 | ||||||
主題 | 465 | |||||
主題Scheme | NDC | |||||
学位名 | ||||||
学位名 | 修士(生命科学) | |||||
学位 | ||||||
値 | master | |||||
研究科・専攻 | ||||||
新領域創成科学研究科生命科学研究系先端生命科学専攻 | ||||||
学位授与年月日 | ||||||
学位授与年月日 | 2011-03-24 | |||||
学位記番号 | ||||||
修創域第3788号 |