{"created":"2021-03-01T07:00:17.593046+00:00","id":40642,"links":{},"metadata":{"_buckets":{"deposit":"bd2c212a-5135-482d-8abf-b78660d562d4"},"_deposit":{"id":"40642","owners":[],"pid":{"revision_id":0,"type":"depid","value":"40642"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00040642","sets":["40:6494:7162:7180:7181","9:504:7165:7182:7183"]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"63","bibliographicPageStart":"58","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"Coastal marine science"}]}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pseudo-nitzschia pungens is a chain forming cosmopolitan marine pennate diatom. It is known to produce the neurotoxin, domoic acid (DA) which is responsible for amnesic shellfish poisoning (ASP). In this study, clonal cultures of P. pungens were established from Sabah and Sarawak. To examine the genetic diversity of P. pungens, the ITS region of rDNA was amplified. Nucleotide sequences obtained were used in secondary structure modeling based on homologous modeling and free energy minimization. The ITS sequences of P. pungens showed common structure of ITS1 and ITS2 transcripts. Universal motifs were found in the ITS2 transcript with pyrimidine-pyrimidine (U-U) mismatch and AAA motif between Helix II-III and UGGU motif in the Helix III. Unambiguous orthologous structural sequence alignment was subsequently used in genetic population analyses. Seven haplotypes were found in the Borneon populations. Kuching haplotype is exclusive from the six haplotypes found in Kudat population. The Kuching and Kudat strains formed two distinctive groups inferred by the Profile Neigboring Joining (PNJ) tree. High FST value (>0.69) suggested no gene flow among the populations of P. pungens in Sabah and Sarawak.","subitem_description_type":"Abstract"}]},"item_4_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Proceedings of the Horiba International Conference \"New Direction of Ocean Research in the Western Pacific\" : Past, Present and Future of UNESCO/IOC/WESTPAC Activity for 50 years and the JSPS Project \"Coastal Marine Science\"","subitem_description_type":"Other"},{"subitem_description":"Section I: Research Articles/Harmful microalgae","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15083/00040633","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"International Coastal Research Center, Atmosphere and Ocean Research Institute, the University of Tokyo"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11957899","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13493000","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"452","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"東京大学大気海洋研究所国際沿岸海洋研究センター"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Faculty of Resource Science and Technology, Universiti Malaysia Sarawak"},{"subitem_text_value":"Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lim, Hong Chang"}],"nameIdentifiers":[{"nameIdentifier":"93380","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Lim, Po-Teen"}],"nameIdentifiers":[{"nameIdentifier":"93381","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Su, Suriyanti Nyun Pau"}],"nameIdentifiers":[{"nameIdentifier":"93382","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Teng, Sing Tung"}],"nameIdentifiers":[{"nameIdentifier":"93383","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Leaw, Chui-Pin"}],"nameIdentifiers":[{"nameIdentifier":"93384","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-14"}],"displaytype":"detail","filename":"CMS350108.pdf","filesize":[{"value":"863.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CMS350108.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/40642/files/CMS350108.pdf"},"version_id":"9bba5b97-8201-4a1a-86c1-7d3fb702889f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Pseudo-nitzschia pungens","subitem_subject_scheme":"Other"},{"subitem_subject":"ITS","subitem_subject_scheme":"Other"},{"subitem_subject":"rDNA","subitem_subject_scheme":"Other"},{"subitem_subject":"population genetic","subitem_subject_scheme":"Other"},{"subitem_subject":"haplotype","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genetic diversity of Pseudo-nitzschia pungens (Bacillariophyceae) in Borneo, Malaysia","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genetic diversity of Pseudo-nitzschia pungens (Bacillariophyceae) in Borneo, Malaysia"}]},"item_type_id":"4","owner":"1","path":["7183","7181"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-05-25"},"publish_date":"2012-05-25","publish_status":"0","recid":"40642","relation_version_is_last":true,"title":["Genetic diversity of Pseudo-nitzschia pungens (Bacillariophyceae) in Borneo, Malaysia"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:15:57.112113+00:00"}