{"created":"2021-03-01T07:00:22.316032+00:00","id":40711,"links":{},"metadata":{"_buckets":{"deposit":"699b1e9b-3d97-4bb2-a2ef-711d66d2b87b"},"_deposit":{"id":"40711","owners":[],"pid":{"revision_id":0,"type":"depid","value":"40711"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00040711","sets":["40:6494:7162:7188:7189","9:504:7165:7190:7191"]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-08-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"45","bibliographicPageStart":"39","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"Coastal marine science"}]}]},"item_4_description_13":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transformation of a cell envelope component of bacteria, lipopolysaccharide (LPS), from bacterial cells into a dissolved form was investigated during time course experiments using seawater culture in conjunction with determining population dynamics of three microbial groups, which are flagellates, bacteria, and viruses. The surface seawater collected from a coastal site was enriched with glutamic acid, and then incubated. LPS concentration was determined using the Limulus amebocyte lysate assay. In the early period of incubation, LPS in the particulate fraction (>2m μm) increased to the maximum in association with bacterial abundance; thereafter, both decreased together. During the fluctuation of LPS in the particulate fraction, LPS in the dissolved fraction (>2m μ) also increased with viral abundance, but then decreased after flagellate abundance began to increase. However, in a culture condition without flagellates, LPS in both dissolved and particulate fractions increased continuously in association with viral abundance. This increase in LPS in the two fractions associated with viral abundance continued while bacterial abundance was maintained at a similar level between the middle and late incubation periods. The temporal change in LPS in the two fractions and its coupled microbial dynamics indicate that LPS in bacterial cells was transformed into nonliving dissolved and particulate forms by viral lysis and that thereafter the LPS was removed through ingestion by flagellates. Thus, our results suggest that the fate of the bacterial cell envelop component, LPS, that is sequentially mediated by viruses and flagellates in the sea.","subitem_description_type":"Abstract"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15083/00040702","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"International Coastal Research Center, Ocean Research Institute, the University of Tokyo"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11957899","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13493000","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"452","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"東京大学海洋研究所国際沿岸海洋研究センター"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Ocean Research Institute, The University of Tokyo, Japan"},{"subitem_text_value":"Faculty of Engineering, Soka University, Japan"},{"subitem_text_value":"Faculty of Education and Human Sciences, Yokohama National University, Japan"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SHIBATA, Akira"}],"nameIdentifiers":[{"nameIdentifier":"139264","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"YASUI, Hirohiko"}],"nameIdentifiers":[{"nameIdentifier":"139265","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"FUKUDA, Hideki"}],"nameIdentifiers":[{"nameIdentifier":"139266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"OGAWA, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"139267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KIKUCHI, Tomohiko"}],"nameIdentifiers":[{"nameIdentifier":"139268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TODA, Tatsuki"}],"nameIdentifiers":[{"nameIdentifier":"139269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TAGUCHI, Satoru"}],"nameIdentifiers":[{"nameIdentifier":"139270","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-27"}],"displaytype":"detail","filename":"CMS330105.pdf","filesize":[{"value":"196.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CMS330105.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/40711/files/CMS330105.pdf"},"version_id":"abcea522-d523-4362-9b21-bebe45de19f7"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Fate of the bacterial cell envelope component, lipopolysaccharide, that is sequentially mediated by viruses and flagellates","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Fate of the bacterial cell envelope component, lipopolysaccharide, that is sequentially mediated by viruses and flagellates"}]},"item_type_id":"4","owner":"1","path":["7191","7189"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-05-28"},"publish_date":"2010-05-28","publish_status":"0","recid":"40711","relation_version_is_last":true,"title":["Fate of the bacterial cell envelope component, lipopolysaccharide, that is sequentially mediated by viruses and flagellates"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:16:18.978517+00:00"}