{"created":"2021-03-01T07:00:23.205772+00:00","id":40724,"links":{},"metadata":{"_buckets":{"deposit":"c4acd757-49b0-4d7c-a9fa-22766dd31740"},"_deposit":{"id":"40724","owners":[],"pid":{"revision_id":0,"type":"depid","value":"40724"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00040724","sets":["40:6494:7162:7192:7193","9:504:7165:7194:7195"]},"item_4_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-08-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"66","bibliographicPageStart":"57","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"Coastal marine science"}]}]},"item_4_description_13":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"There is still concern about the effects of organotin compounds (OTs) on marine organisms, and especially on their reproductive systems. We investigated the toxicity of tributyltin oxide (TBTO) on spermatogenesis in a marine fish, the mummichog, Fundulus heteroclitus. The primary purpose of this study is to find biomarkers for evaluation of the effects of TBTO on fish spermatogenesis. We used cDNA subtraction and differential display methods to clone genes differentially expressed in the mummichog testis by TBTO exposure. Six downregulated genes have been identified to date. A GenBank homology search revealed that these genes were homologous to the arginase II gene, a testicular type of creatine kinase gene, two different types of dynein heavy-chain genes, a mitochondrial carrier gene, and a functionally unidentified gene. Real-time PCR revealed that exposure of the fish to 4m g/L of TBTO significantly reduced expression of at least three of the six genes: those encoding creatine kinase, a dynein heavy chain, and the functionally unidentified gene in the testis. In the 4m g/L exposure group, significant reduction in spermatogenic cell proliferation and significant increase in numbers of apoptotic germ cells in the testis were observed. These genes can therefore be used as biomarkers for evaluating the effects of TBTO on fish spermatogenesis.","subitem_description_type":"Abstract"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15083/00040715","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"International Coastal Research Center, Ocean Research Institute, the University of Tokyo"}]},"item_4_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11957899","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13493000","subitem_source_identifier_type":"ISSN"}]},"item_4_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"452","subitem_subject_scheme":"NDC"}]},"item_4_text_21":{"attribute_name":"出版者別名","attribute_value_mlt":[{"subitem_text_value":"東京大学海洋研究所国際沿岸海洋研究センター"}]},"item_4_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Fisheries Research Agency, National Research Institute of Fisheries and Environment of Inland Sea"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MOCHIDA, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"139324","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ITO, Katsutoshi"}],"nameIdentifiers":[{"nameIdentifier":"139325","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ONDUKA, Toshimitsu"}],"nameIdentifiers":[{"nameIdentifier":"139326","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"KAKUNO, Akira"}],"nameIdentifiers":[{"nameIdentifier":"139327","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"FUJII, Kazunori"}],"nameIdentifiers":[{"nameIdentifier":"139328","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-27"}],"displaytype":"detail","filename":"CMS320109.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"CMS320109.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/40724/files/CMS320109.pdf"},"version_id":"f3f9ea3b-6723-4666-bea8-2204fa1d335d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"bromodeoxyuridine","subitem_subject_scheme":"Other"},{"subitem_subject":"cDNA subtraction","subitem_subject_scheme":"Other"},{"subitem_subject":"differential display","subitem_subject_scheme":"Other"},{"subitem_subject":"real-time PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"teleost","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genes differentially expressed in testis of a marine fish, the mummichog (Fundulus heteroclitus) after exposure to tributyltin oxide","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genes differentially expressed in testis of a marine fish, the mummichog (Fundulus heteroclitus) after exposure to tributyltin oxide"}]},"item_type_id":"4","owner":"1","path":["7195","7193"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-02-18"},"publish_date":"2009-02-18","publish_status":"0","recid":"40724","relation_version_is_last":true,"title":["Genes differentially expressed in testis of a marine fish, the mummichog (Fundulus heteroclitus) after exposure to tributyltin oxide"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T04:16:20.120975+00:00"}