{"created":"2021-03-01T06:20:44.040495+00:00","id":4078,"links":{},"metadata":{"_buckets":{"deposit":"cfa37ec1-e19c-4cbd-821d-86005546fec3"},"_deposit":{"id":"4078","owners":[],"pid":{"revision_id":0,"type":"depid","value":"4078"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00004078","sets":["125:355:356","9:233:280"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ヒト冠動脈平滑筋におけるSerum Amyloid A及びLysophosphatidylcholineの生理作用 : TRPチャネルの役割"}]},"item_7_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-03-24","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_7_date_granted_25":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2011-03-24"}]},"item_7_degree_grantor_23":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"University of Tokyo (東京大学)"}]}]},"item_7_degree_name_20":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Serum amyloid A (SAA) and lysophosphatidylcholine (LPC) contribute to physiological processes of atherosclerosis and cardiovascular disease. However, the effects of SAA/LPC on human coronary artery smooth muscle cells (hCASMCs) have not been investigated. Therefore, I examined the effects of SAA/LPC on Ca2+/Mg2+ mobilization and its underlying mechanisms in hCASMCs. Methods: Intracellular Ca2+/Mg2+ concentration ([Ca2+]i/[Mg2+]i) was measured with fura-2 AM/mag-fura-2 AM. The conventional/real-time quantitative RT-PCR, Western blot and immunocytochemistry were applied. Small interfering RNA (siRNA) for TRPC4 was used to knock down TRPC4. Result: SAA/LPC increased [Ca2+]i by Ca2+ entry. The SAA-induced Ca2+ entry was inhibited by Gd3+, SKF96365 and 2-aminoethoxydiphenyl borate (2-APB), but not nifedipine. The LPC-induced Ca2+ entry was blocked by Gd3+, but not nifedipine, SKF96365 and 2-APB. U73122 and PTX prevented the activation of SAA-, but not LPC-induced Ca2+ influx. LPC, but not SAA, increased [Mg2+]i. The RT-PCR, Western blot and immunocytochemistry of TRP channels revealed the expression of TRPC1/4, TRPV1/2/4 and TRPM7. In siRNA treated cells, the level of TRPC4 mRNA was reduced, and Ca2+ response for SAA was attenuated, compared with control cells. Conclusion: These results suggest that SAA/LPC activate Ca2+ influx in hCASMCs; SAA activates it via PTX-sensitive G-protein, PLC and TRPC pathways, where TRPC4 may be involved. On the other hand, LPC may activate it independently of these pathways. TRP protein may be a target molecule of Ca2+ signaling in hCASMCs elicited by SAA/LPC, which may play roles in coronary muscle remodeling under the pathophysiological conditions such as atherosclerosis.","subitem_description_type":"Abstract"}]},"item_7_dissertation_number_26":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第27057号"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"9400","nameIdentifierScheme":"WEKO"}],"names":[{"name":"田中, 悌史"}]}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15083/00004069","subitem_identifier_reg_type":"JaLC"}]},"item_7_select_21":{"attribute_name":"学位","attribute_value_mlt":[{"subitem_select_item":"doctoral"}]},"item_7_subject_13":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_7_text_22":{"attribute_name":"学位分野","attribute_value_mlt":[{"subitem_text_value":"Medical Science(医学)"}]},"item_7_text_24":{"attribute_name":"研究科・専攻","attribute_value_mlt":[{"subitem_text_value":"Department of Internal Medicine, Graduate School of Medicine (医学系研究科内科学専攻)"}]},"item_7_text_27":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_value":"博医第3667号"}]},"item_7_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"東京大学大学院医学系研究科内科学専攻"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tanaka, Tomofumi"}],"nameIdentifiers":[{"nameIdentifier":"9399","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-01"}],"displaytype":"detail","filename":"h22_tanaka.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"h22_tanaka.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/4078/files/h22_tanaka.pdf"},"version_id":"b70c595a-47c2-4c60-b2d1-027ef49972e1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Effects of Serum Amyloid A and Lysophosphatidylcholine on Human Coronary Artery Smooth Muscle Cells : Roles of Transient Receptor Potential Channels","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of Serum Amyloid A and Lysophosphatidylcholine on Human Coronary Artery Smooth Muscle Cells : Roles of Transient Receptor Potential Channels"}]},"item_type_id":"7","owner":"1","path":["280","356"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-08"},"publish_date":"2012-11-08","publish_status":"0","recid":"4078","relation_version_is_last":true,"title":["Effects of Serum Amyloid A and Lysophosphatidylcholine on Human Coronary Artery Smooth Muscle Cells : Roles of Transient Receptor Potential Channels"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:45:50.512483+00:00"}