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cDNA\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3068\u305d\u306e\u767a\u73fe\u306b\u3064\u3044\u3066\u89e3\u6790\u3092\u884c\u3063\u305f.ICR\u30de\u30a6\u30b9\u5375\u5de3\u306ecDNA\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u304b\u3089\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3055\u308c\u305f\u30de\u30a6\u30b920\u03b1-HSD cDNA\u306f323\u500b\u306e\u30a2\u30df\u30ce\u9178\u3092\u30b3\u30fc\u30c9\u3057,\u30e9\u30c3\u30c820\u03b1-HSD cDNA\u306893%\u306e\u76f8\u540c\u6027\u3092\u6709\u3057\u3066\u3044\u305f.\u30de\u30a6\u30b920\u03b1-HSD\u306e\u30a2\u30df\u30ce\u9178\u914d\u5217\u306b\u306f\u30a2\u30eb\u30c9\u30fb\u30b1\u30c8\u9084\u5143\u5316\u9175\u7d20\u3068\u3057\u3066\u306e\u6d3b\u6027\u767a\u73fe\u306b\u5fc5\u9808\u306a\u88dc\u9175\u7d20\u7d50\u5408\u90e8\u4f4d\u3092\u69cb\u6210\u3059\u308b4\u500b\u306e\u30a2\u30df\u30ce\u9178(Asp50,Tyr55,Lys84,His117)\u304c\u4fdd\u5b58\u3055\u308c\u3066\u3044\u305f.\u307e\u305f,\u30de\u30a6\u30b920\u03b1-HSD mRNA\u306fprogesterone\u6fc3\u5ea6\u306e\u4f4e\u4e0b\u306820\u03b1-OHP\u6fc3\u5ea6\u306e\u4e0a\u6607\u304c\u898b\u3089\u308c\u308b\u5206\u5a29\u524d\u5f8c\u306e\u5375\u5de3\u306b\u304a\u3044\u3066\u8457\u3057\u3044\u767a\u73fe\u306e\u4e0a\u6607\u304c\u8a8d\u3081\u3089\u308c,\u307e\u305f\u7d44\u7e54\u5b66\u7684\u306b\u3082\u3053\u306e\u6642\u671f\u306e\u9ec4\u4f53\u7d30\u80de\u3067\u767a\u73fe\u304c\u78ba\u8a8d\u3055\u308c\u305f.\u3055\u3089\u306b,\u598a\u5a20\u671f\u5b50\u5bae\u5185\u7d44\u7e54\u306b\u304a\u3044\u3066\u306f,in situ hybridization\u6cd5\u306b\u3088\u308a\u5b50\u5bae\u5185\u819c\u4e0a\u76ae\u7d30\u80de\u3084\u80ce\u76e4,\u80ce\u5b50\u8868\u76ae\u306720\u03b1-HSD mRNA\u304c\u691c\u51fa\u3055\u308c,\u307e\u305fNorthern hybridization\u6cd5\u306b\u3088\u308a\u80ce\u76e4\u3067\u306f10\u65e5\u9803\u306b,\u5b50\u5bae\u3067\u306f10\u65e5\u76ee\u304b\u3089\u5206\u5a29\u65e5\u306e18\u65e5\u76ee\u307e\u3067\u691c\u51fa\u3055\u308c16\u65e5\u306b\u30d4\u30fc\u30af\u306b\u9054\u3057\u305f.\u3053\u308c\u3089\u306e\u7d50\u679c\u306f,20\u03b1-HSD\u304c\u80ce\u5b50\u767a\u80b2\u74b0\u5883\u3067\u306e\u9ad8\u6fc3\u5ea6progesterone\u306e\u4f5c\u7528\u3092\u7de9\u548c\u3057,\u80ce\u5b50\u306e\u6b63\u5e38\u306a\u767a\u80b2\u3092\u7dad\u6301\u3059\u308b\u6a5f\u80fd\u3092\u6709\u3059\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b.\u3000\u7b2c2\u7ae0\u306b\u304a\u3044\u3066\u306f,20\u03b1-HSD\u907a\u4f1d\u5b50KO\u30de\u30a6\u30b9\u4f5c\u88fd\u306e\u305f\u3081\u306e\u30bf\u30fc\u30b2\u30c6\u30a3\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u69cb\u7bc9\u306e\u305f\u3081,\u30de\u30a6\u30b920\u03b1-HSD cDNA\u3092\u3082\u3068\u306b129\u7cfb\u7d71\u306e\u30b2\u30ce\u30e0\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3057\u305f.\u5f97\u3089\u308c\u305f5\u3064\u306e\u30b2\u30ce\u30e0\u30af\u30ed\u30fc\u30f3\u3092\u7d71\u5408\u3057\u305f\u7d50\u679c,20\u03b1-HSD\u30b2\u30ce\u30e0DNA\u306f,\u30a8\u30af\u30bd\u30f31\u304b\u30899\u307e\u3067\u7d0418kb\u306b\u6e21\u308a,\u5404\u30a8\u30af\u30bd\u30f3\u306e\u30b5\u30a4\u30ba\u304c\u30e9\u30c3\u30c8,\u30d2\u30c8\u306e20\u03b1-HSD\u907a\u4f1d\u5b50\u3068\u3088\u304f\u4e00\u81f4\u3059\u308b\u3068\u3068\u3082\u306b,\u30b2\u30ce\u30e0\u69cb\u9020\u3082\u30d2\u30c820\u03b1-HSD\u907a\u4f1d\u5b50\u3068\u985e\u4f3c\u3057\u3066\u3044\u305f.\u307e\u305f20\u03b1-HSD\u306e\u6d3b\u6027\u767a\u73fe\u306b\u5fc5\u9808\u3067\u3042\u308b4\u500b\u306e\u30a2\u30df\u30ce\u9178\u304c\u30a8\u30af\u30bd\u30f32\u304b\u30894\u306b\u30b3\u30fc\u30c9\u3055\u308c\u3066\u3044\u305f\u3053\u3068\u304b\u3089,\u30bf\u30fc\u30b2\u30c6\u30a3\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u306f\u3053\u306e\u9818\u57df\u3092\u6a19\u7684\u90e8\u4f4d\u306b\u5b9a\u3081\u3066\u30cd\u30aa\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\u3067\u7f6e\u63db\u3059\u308b\u3068\u3068\u3082\u306b,20\u03b1-HSD\u306e\u767a\u73fe\u90e8\u4f4d\u3092KO\u30de\u30a6\u30b9\u306b\u304a\u3044\u3066\u691c\u51fa\u3067\u304d\u308b\u3088\u3046\u306b\u30a8\u30af\u30bd\u30f32\u306e\u9014\u4e2d\u306binternal 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