WEKO3
AND
アイテム
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Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate
http://hdl.handle.net/2261/37930
162ea6d4-32b0-43f0-a5b3-04c2d5edf639
名前 / ファイル | ライセンス | アクション | |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-11-02 | |||||
タイトル | ||||||
タイトル | Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | aspartate isomerization | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | isoaspartate | |||||
主題Scheme | Other | |||||
キーワード | ||||||
主題 | succinimide | |||||
主題Scheme | Other | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Noguchi, Shuji
× Noguchi, Shuji |
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著者別名 | ||||||
識別子 | ||||||
識別子 | 1712 | |||||
識別子Scheme | WEKO | |||||
姓名 | ||||||
姓名 | 野口, 修治 | |||||
著者所属 | ||||||
著者所属 | Graduate School of Pharmaceutical Sciences, The University of Tokyo | |||||
著者所属 | ||||||
著者所属 | 東京大学大学院薬学系研究科 蛋白構造生物学教室 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Aspartates in proteins are isomerized non-enzymatically to isoaspartate via succinimide in vitro and in vivo. In order to elucidate the mechanism of isoaspartate formation within the Asp45-Glu46 sequence of Ustilago sphaerogena ribonuclease U2 based on three-dimensional structure, crystal structures of ribonuclease U2 complexed with adenosine 3'-monophosphate have been solved at 0.96 and 0.99 Å resolution. The crystal structures revealed that the C atom of Asp45 is located just beside the main-chain N atom of Glu46 and that the conformation which is suitable for succinimide formation is stabilized by a hydrogen-bond network mediated by water molecules 190, 219 and 220. These water molecules are suggested to promote the formation of isoaspartate via succinimide: in the succinimide-formation reaction water 219 receives a proton from the N atom of Glu46 as a general base and waters 190 and 220 stabilize the tetrahedral intermediate, and in the succinimide-hydrolysis reaction water 219 provides a proton for the N atom of Glu46 as a general acid. The purine-base recognition scheme of ribonuclease U2 is also discussed. | |||||
書誌情報 |
Acta crystallographica. Sect. D, Biological crystallography 巻 66, 号 7, p. 843-849, 発行日 2010-07 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09074449 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885500 | |||||
DOI | ||||||
関連識別子 | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1107/S0907444910019621 | |||||
権利 | ||||||
権利情報 | Copyright International Union of Crystallography | |||||
日本十進分類法 | ||||||
主題 | 499 | |||||
主題Scheme | NDC | |||||
出版者 | ||||||
出版者 | International Union of Crystallography |