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  1. 120 薬学系研究科・薬学部
  2. 20 機能薬学専攻
  3. 1202010 学術雑誌論文
  1. 0 資料タイプ別
  2. 10 学術雑誌論文
  3. 014 自然科学

Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate

http://hdl.handle.net/2261/37930
162ea6d4-32b0-43f0-a5b3-04c2d5edf639
名前 / ファイル ライセンス アクション
snoguhi_u2-3AMP_ActaD.pdf snoguhi_u2-3AMP_ActaD.pdf (1.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2010-11-02
タイトル
タイトル Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate
言語
言語 eng
キーワード
主題 aspartate isomerization
主題Scheme Other
キーワード
主題 isoaspartate
主題Scheme Other
キーワード
主題 succinimide
主題Scheme Other
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Noguchi, Shuji

× Noguchi, Shuji

WEKO 1711

Noguchi, Shuji

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著者別名
識別子
識別子 1712
識別子Scheme WEKO
姓名
姓名 野口, 修治
著者所属
著者所属 Graduate School of Pharmaceutical Sciences, The University of Tokyo
著者所属
著者所属 東京大学大学院薬学系研究科 蛋白構造生物学教室
抄録
内容記述タイプ Abstract
内容記述 Aspartates in proteins are isomerized non-enzymatically to isoaspartate via succinimide in vitro and in vivo. In order to elucidate the mechanism of isoaspartate formation within the Asp45-Glu46 sequence of Ustilago sphaerogena ribonuclease U2 based on three-dimensional structure, crystal structures of ribonuclease U2 complexed with adenosine 3'-monophosphate have been solved at 0.96 and 0.99 Å resolution. The crystal structures revealed that the C atom of Asp45 is located just beside the main-chain N atom of Glu46 and that the conformation which is suitable for succinimide formation is stabilized by a hydrogen-bond network mediated by water molecules 190, 219 and 220. These water molecules are suggested to promote the formation of isoaspartate via succinimide: in the succinimide-formation reaction water 219 receives a proton from the N atom of Glu46 as a general base and waters 190 and 220 stabilize the tetrahedral intermediate, and in the succinimide-hydrolysis reaction water 219 provides a proton for the N atom of Glu46 as a general acid. The purine-base recognition scheme of ribonuclease U2 is also discussed.
書誌情報 Acta crystallographica. Sect. D, Biological crystallography

巻 66, 号 7, p. 843-849, 発行日 2010-07
ISSN
収録物識別子タイプ ISSN
収録物識別子 09074449
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10885500
DOI
関連識別子
識別子タイプ DOI
関連識別子 info:doi/10.1107/S0907444910019621
権利
権利情報 Copyright International Union of Crystallography
日本十進分類法
主題 499
主題Scheme NDC
出版者
出版者 International Union of Crystallography
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