{"created":"2021-03-01T06:17:02.469307+00:00","id":500,"links":{},"metadata":{"_buckets":{"deposit":"6c6a79db-ccbd-4dd9-a7af-e43d2f53097c"},"_deposit":{"id":"500","owners":[],"pid":{"revision_id":0,"type":"depid","value":"500"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00000500","sets":["20:119:120","9:10:11"]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"849","bibliographicPageStart":"843","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"Acta crystallographica. Sect. D, Biological crystallography"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aspartates in proteins are isomerized non-enzymatically to isoaspartate via succinimide in vitro and in vivo. In order to elucidate the mechanism of isoaspartate formation within the Asp45-Glu46 sequence of Ustilago sphaerogena ribonuclease U2 based on three-dimensional structure, crystal structures of ribonuclease U2 complexed with adenosine 3'-monophosphate have been solved at 0.96 and 0.99 Å resolution. The crystal structures revealed that the C atom of Asp45 is located just beside the main-chain N atom of Glu46 and that the conformation which is suitable for succinimide formation is stabilized by a hydrogen-bond network mediated by water molecules 190, 219 and 220. These water molecules are suggested to promote the formation of isoaspartate via succinimide: in the succinimide-formation reaction water 219 receives a proton from the N atom of Glu46 as a general base and waters 190 and 220 stabilize the tetrahedral intermediate, and in the succinimide-hydrolysis reaction water 219 provides a proton for the N atom of Glu46 as a general acid. The purine-base recognition scheme of ribonuclease U2 is also discussed.","subitem_description_type":"Abstract"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"1712","nameIdentifierScheme":"WEKO"}],"names":[{"name":"野口, 修治"}]}]},"item_2_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"International Union of Crystallography"}]},"item_2_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1107/S0907444910019621","subitem_relation_type_select":"DOI"}}]},"item_2_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright International Union of Crystallography"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10885500","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09074449","subitem_source_identifier_type":"ISSN"}]},"item_2_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"499","subitem_subject_scheme":"NDC"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Pharmaceutical Sciences, The University of Tokyo"},{"subitem_text_value":"東京大学大学院薬学系研究科 蛋白構造生物学教室"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Noguchi, Shuji"}],"nameIdentifiers":[{"nameIdentifier":"1711","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-30"}],"displaytype":"detail","filename":"snoguhi_u2-3AMP_ActaD.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"snoguhi_u2-3AMP_ActaD.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/500/files/snoguhi_u2-3AMP_ActaD.pdf"},"version_id":"dae717ac-8274-48ed-b62c-bc3e061389d1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"aspartate isomerization","subitem_subject_scheme":"Other"},{"subitem_subject":"isoaspartate","subitem_subject_scheme":"Other"},{"subitem_subject":"succinimide","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate"}]},"item_type_id":"2","owner":"1","path":["11","120"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-11-02"},"publish_date":"2010-11-02","publish_status":"0","recid":"500","relation_version_is_last":true,"title":["Isomerization mechanism of aspartate to isoaspartate implied by structures of Ustilago sphaerogena ribonuclease U2 complexed with adenosine 3'-monophosphate"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:41:27.938685+00:00"}