{"created":"2021-03-01T06:17:03.075650+00:00","id":511,"links":{},"metadata":{"_buckets":{"deposit":"a0b84331-3108-48f3-8e76-8de07ded3f8d"},"_deposit":{"id":"511","owners":[],"pid":{"revision_id":0,"type":"depid","value":"511"},"status":"published"},"_oai":{"id":"oai:repository.dl.itc.u-tokyo.ac.jp:00000511","sets":["20:119:120","9:10:11"]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"1561","bibliographicPageStart":"1559","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"Protein and peptide letters"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Asparagine can be non-enzymatically deamidated and isomerized via succinimide to isoaspartate. Thispost-translational modification can potentially alter the physical properties or the function of the parent protein.Asn32 of ribonuclease U2A from Ustilago sphaerogena is known to rapidly deamidate and isomerize in alkalineconditions. The crystal structure of ribonuclease U2A complexed with 2'-adenylic acid and calcium ions wasdetermined at 1.03 Å resolution. In this structure, the region from Asp29 to Asp37 winds around a calcium ion, andthe main-chain of Asn32–Gly33 adopts an extended conformation. Rotation of the side-chain of Asn32 could bringAsn32Cγ into close proximity to Gly33N, in a conformation suitable for succinimide formation. The structuresuggests that in solution the region around Asn32–Gly33 is likely to be in equilibrium between multiple conformers,with the deamidation of Asn32 proceeding when the region adopts an extended conformation.","subitem_description_type":"Abstract"}]},"item_2_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"pre-print版(pre-refereeing)","subitem_description_type":"Other"}]},"item_2_publisher_20":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Bentham Science Publishers"}]},"item_2_relation_25":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.bentham.org/ppl/index.htm","subitem_relation_type_select":"URI"}}]},"item_2_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Bentham Science Publishers"}]},"item_2_select_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_2_source_id_10":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1102463X","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_8":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09298665","subitem_source_identifier_type":"ISSN"}]},"item_2_subject_15":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"499","subitem_subject_scheme":"NDC"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Pharmaceutical Sciences, The University of Tokyo"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Noguchi, Shuji"}],"nameIdentifiers":[{"nameIdentifier":"1729","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-05-30"}],"displaytype":"detail","filename":"PPL_17_1559.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PPL_17_1559.pdf","url":"https://repository.dl.itc.u-tokyo.ac.jp/record/511/files/PPL_17_1559.pdf"},"version_id":"4d3de7ee-18c7-4272-85c4-17745f6496fd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Asparagine deamidation","subitem_subject_scheme":"Other"},{"subitem_subject":"Isoaspartate","subitem_subject_scheme":"Other"},{"subitem_subject":"Isomerization","subitem_subject_scheme":"Other"},{"subitem_subject":"Ribonuclease U2","subitem_subject_scheme":"Other"},{"subitem_subject":"Succinimide","subitem_subject_scheme":"Other"},{"subitem_subject":"Ustilago sphaerogena","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Conformational Variation Revealed by the Crystal Structure of RNase U2A Complexed with Ca Ion and 2'-Adenylic Acid at 1.03 Å Resolution","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Conformational Variation Revealed by the Crystal Structure of RNase U2A Complexed with Ca Ion and 2'-Adenylic Acid at 1.03 Å Resolution"}]},"item_type_id":"2","owner":"1","path":["11","120"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-01-04"},"publish_date":"2011-01-04","publish_status":"0","recid":"511","relation_version_is_last":true,"title":["Conformational Variation Revealed by the Crystal Structure of RNase U2A Complexed with Ca Ion and 2'-Adenylic Acid at 1.03 Å Resolution"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-19T03:41:31.104933+00:00"}